PXD017232

PXD017232 is an original dataset announced via ProteomeXchange.

Title	Agonists of orally expressed TRP channels stimulate salivary secretion and modify the salivary proteome
Description	Natural compounds that can stimulate salivary secretion are of interest in developing treatments for xerostomia, the perception of a dry mouth, that affects between 10 and 30% of the adult and elderly population. Chemesthetic transient receptor potential (TRP) channels are expressed in the surface of the oral mucosa. The TRPV1 agonists capsaicin and piperine have been shown to increase salivary flow when introduced into the oral cavity but the sialogogic properties of other TRP channel agonists have not been investigated. In this study we have determined the influence of different TRP channel agonists on the flow and protein composition of saliva. Mouth rinsing with the TRPV1 agonist nonivamide or menthol, a TRPM8 agonist, increased whole mouth saliva (WMS) flow and total protein secretion compared to unstimulated saliva, the vehicle control mouth rinse or cinnamaldehyde, a TRPA1 agonist. Nonivamide also increased the flow of labial minor gland saliva but parotid saliva flow rate was not increased. The influence of TRP channel agonists on the composition and function of the salivary proteome was investigated using a multi-batch quantitative mass spectrometry method novel to salivary proteomics. Inter-personal and inter-mouth rinse variation was observed in the secreted proteomes and, using a novel bioinformatics method, inter-day variation was identified with some of the mouth rinses. Significant changes in specific salivary proteins were identified after all mouth rinses. In the case of nonivamide, these changes were attributed to functional shifts in the WMS secreted, primarily the over representation of salivary and non-salivary cystatins which was confirmed by immunoassay. This study provides new evidence of the impact of TRP channel agonists on the salivary proteome and the stimulation of salivary secretion by a TRPM8 channel agonist, which suggests that TRP channel agonists are potential candidates for developing treatments for sufferers of xerostomia
HostingRepository	PRIDE
AnnounceDate	2020-07-16
AnnouncementXML	Submission_2020-07-16_03:46:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack Houghton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-21 05:57:27	ID requested
⏵ 1	2020-07-16 03:46:58	announced

Houghton JW, Carpenter G, Hans J, Pesaro M, Lynham S, Proctor G, Agonists of Orally Expressed TRP Channels Stimulate Salivary Secretion and Modify the Salivary Proteome. Mol Cell Proteomics, 19(10):1664-1676(2020) [pubmed]

submitter keyword: Saliva, Transient Receptor Potential Channel Agonists, Proteome, Nonivamide, Cinnamaldehyde, Menthol

Gordon Proctor
contact affiliation	Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London (London, UK)
contact email	gordon.proctor@kcl.ac.uk
lab head
Jack Houghton
contact affiliation	King's College London
contact email	jack.houghton@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017232
     1. Label: PRIDE project
     2. Name: Agonists of orally expressed TRP channels stimulate salivary secretion and modify the salivary proteome