PXD017230

PXD017230 is an original dataset announced via ProteomeXchange.

Title	HDAC3 preferentially binds to RUNX1 in t(8;21) AML cells and collaborates with RUNX1 to repress AML1-ETO-mediated transcriptional activation
Description	Human histone deacetylase 3 (HDAC3) plays an important role in gene transcription in diseased human cells, such as leukemia. The t(8;21) chromosomal translocation is one of the most commonly observed genetic abnormalities associated with acute myeloid leukemia. This translocation generates the AML1-ETO fusion protein between the wild-type RUNX1 transcription factor and wild-type ETO transcriptional corepressor. To better understand the role of HDAC3 in t(8;21) leukemogenesis, the human HDAC3-containing complexes were isolated from stably-transfected HeLa cells by using anti-FLAG immunoprecipitation. The resulting complexes were resolved in SDS-PAGE. The components of the complexes were identified using LC-MS/MS. We report here that the human RUNX1 transcription is a component of the HDAC3 complexes. We demonstrate that HDAC3 and RUNX1 collaboratively repress AML1-ETO-mediated transcription. These results reveal new insight into how AML1-ETO, RUNX1, and HDAC3 crosstalk to deregulate gene transcription in t(8;21) leukemia cells.
HostingRepository	PRIDE
AnnounceDate	2020-02-17
AnnouncementXML	Submission_2020-02-24_02:38:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017230
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Markus Kalkum
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-21 02:17:22	ID requested
1	2020-02-16 23:21:52	announced
⏵ 2	2020-02-24 02:38:15	announced	2020-02-24: Updated publication reference for PubMed record(s): 32071087.

Guo C, Li J, Steinauer N, Wong M, Wu B, Dickson A, Kalkum M, Zhang J, Histone deacetylase 3 preferentially binds and collaborates with the transcription factor RUNX1 to repress AML1-ETO-dependent transcription in t(8;21) AML. J Biol Chem, 295(13):4212-4223(2020) [pubmed]

submitter keyword: AML, leukemia, t(8;21) translocation, HDAC3, RUNX1

Markus Kalkum
contact affiliation	The Beckman Research Institute of the City of Hope
contact email	mkalkum@coh.org
lab head
Markus Kalkum
contact affiliation	City of Hope
contact email	iontrap.ms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017230
     1. Label: PRIDE project
     2. Name: HDAC3 preferentially binds to RUNX1 in t(8;21) AML cells and collaborates with RUNX1 to repress AML1-ETO-mediated transcriptional activation