PXD017215

PXD017215 is an original dataset announced via ProteomeXchange.

Title	A novel polyubiquitin chain linkage formed by viral Ubiquitin resists cleavage by deubiquitinating enzymes
Description	The project reports the structural difference between viral Ubiquitin (vUB) and human ubiquitin, hence causing local and global destabilization in vUB. Viral Ubiquitin conjugates are degraded by proteasome machinery. The in-vitro biochemical assays suggest comparable activity of mono UB and mono-vUB against different set of E2 and E3 enzymes. The project also reports the novel isopeptide linkage in viral ubiquitin at K54 position through MS/MS, which is made by E2D2 and not by other E2s used in this study. Most importantly K54-conjugates were found to be resistant towards deubiquitinase enzymes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:54.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hitendra Negi
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-20 08:23:45	ID requested
1	2020-06-27 16:56:34	announced
⏵ 2	2024-10-22 05:08:02	announced	2024-10-22: Updated project metadata.

10.1042/bcj20200289;

Negi H, Reddy PP, Vengayil V, Patole C, Laxman S, Das R, A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes. Biochem J, 477(12):2193-2219(2020) [pubmed]

submitter keyword: Ubiquitination, DUBs,NMR, LC-MS, Isopeptide bond, Proteasomal degradation

Dr. Ranabir Das
contact affiliation	National Centre for Biological Sciences, Bangalore, India
contact email	rana@ncbs.res.in
lab head
Hitendra Negi
contact affiliation	National Centre for Biological Sciences
contact email	hitendran@ncbs.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017215

PRIDE project URI

• PRIDE
  1. PXD017215
     1. Label: PRIDE project
     2. Name: A novel polyubiquitin chain linkage formed by viral Ubiquitin resists cleavage by deubiquitinating enzymes