PXD017215 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A novel polyubiquitin chain linkage formed by viral Ubiquitin resists cleavage by deubiquitinating enzymes |
Description | The project reports the structural difference between viral Ubiquitin (vUB) and human ubiquitin, hence causing local and global destabilization in vUB. Viral Ubiquitin conjugates are degraded by proteasome machinery. The in-vitro biochemical assays suggest comparable activity of mono UB and mono-vUB against different set of E2 and E3 enzymes. The project also reports the novel isopeptide linkage in viral ubiquitin at K54 position through MS/MS, which is made by E2D2 and not by other E2s used in this study. Most importantly K54-conjugates were found to be resistant towards deubiquitinase enzymes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:07:54.781.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hitendra Negi |
SpeciesList | scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-20 08:23:45 | ID requested | |
1 | 2020-06-27 16:56:34 | announced | |
⏵ 2 | 2024-10-22 05:08:02 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1042/bcj20200289; |
Negi H, Reddy PP, Vengayil V, Patole C, Laxman S, Das R, A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes. Biochem J, 477(12):2193-2219(2020) [pubmed] |
Keyword List
submitter keyword: Ubiquitination, DUBs,NMR, LC-MS, Isopeptide bond, Proteasomal degradation |
Contact List
Dr. Ranabir Das |
contact affiliation | National Centre for Biological Sciences, Bangalore, India |
contact email | rana@ncbs.res.in |
lab head | |
Hitendra Negi |
contact affiliation | National Centre for Biological Sciences |
contact email | hitendran@ncbs.res.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD017215 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD017215
- Label: PRIDE project
- Name: A novel polyubiquitin chain linkage formed by viral Ubiquitin resists cleavage by deubiquitinating enzymes