PXD017208

PXD017208 is an original dataset announced via ProteomeXchange.

Title	Pathogen-pathogen interactions in the microbiome, a way to soothe virulence?
Description	The rise of multi-drug resistance in bacterial pathogens imposes the need to study these organisms from new angles. A little explored outset is to scrutinize bacterial niche adaptations and interactions among pathogenic and commensal bacteria, because they can provide a better understanding of the fitness of pathogens in their human host. We have previously shown that co-culturing of the pathogen Staphylococcus aureus with co-resident Klebsiella oxytoca or Bacillus thuringiensis wound isolates resulted in reduced levels of virulence factor secretion, suggesting that the presence of these co-resident bacteria would modulate S. aureus virulence. In the present study, we performed an in-depth investigation of changes in S. aureus gene expression upon co-cultivation with K. oxytoca and B. thuringiensis under infection-mimicking conditions. To this end, we profiled the cellular proteomes of the co-existing bacteria with special focus on S. aureus. In parallel, we employed RNA sequencing to highlight global changes in staphylococcal behaviour. The results imply that co-colonizing bacteria from chronic wounds can pacify S. aureus, and this conclusion was verified in a Galleria mellonella infection model. Altogether, our findings show that the presence of K. oxytoca and B. thuringiensis leads to massive rearrangements in S. aureus physiology and substantial reduction in virulence.
HostingRepository	PRIDE
AnnounceDate	2020-04-22
AnnouncementXML	Submission_2020-04-21_22:21:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Bacillus thuringiensis; NCBI TaxID: 1428; scientific name: Klebsiella oxytoca; NCBI TaxID: 571; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-20 08:19:24	ID requested
⏵ 1	2020-04-21 22:21:39	announced

Dataset with its publication pending

submitter keyword: Staphylococcus aureus, Klebsiella oxytoca, Galleria mellonella, co-infection, transcriptome, proteome, chronic wound

Dörte Becher
contact affiliation	University of Greifswald Center of Functional Genomics of Microbes Institute of Microbiology Department of Microbial Proteomics Felix-Hausdorff-Str.8 17489 Greifswald
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017208
     1. Label: PRIDE project
     2. Name: Pathogen-pathogen interactions in the microbiome, a way to soothe virulence?