PXD017208 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pathogen-pathogen interactions in the microbiome, a way to soothe virulence? |
Description | The rise of multi-drug resistance in bacterial pathogens imposes the need to study these organisms from new angles. A little explored outset is to scrutinize bacterial niche adaptations and interactions among pathogenic and commensal bacteria, because they can provide a better understanding of the fitness of pathogens in their human host. We have previously shown that co-culturing of the pathogen Staphylococcus aureus with co-resident Klebsiella oxytoca or Bacillus thuringiensis wound isolates resulted in reduced levels of virulence factor secretion, suggesting that the presence of these co-resident bacteria would modulate S. aureus virulence. In the present study, we performed an in-depth investigation of changes in S. aureus gene expression upon co-cultivation with K. oxytoca and B. thuringiensis under infection-mimicking conditions. To this end, we profiled the cellular proteomes of the co-existing bacteria with special focus on S. aureus. In parallel, we employed RNA sequencing to highlight global changes in staphylococcal behaviour. The results imply that co-colonizing bacteria from chronic wounds can pacify S. aureus, and this conclusion was verified in a Galleria mellonella infection model. Altogether, our findings show that the presence of K. oxytoca and B. thuringiensis leads to massive rearrangements in S. aureus physiology and substantial reduction in virulence. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-22 |
AnnouncementXML | Submission_2020-04-21_22:21:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sandra Maass |
SpeciesList | scientific name: Bacillus thuringiensis; NCBI TaxID: 1428; scientific name: Klebsiella oxytoca; NCBI TaxID: 571; scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-20 08:19:24 | ID requested | |
⏵ 1 | 2020-04-21 22:21:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Staphylococcus aureus, Klebsiella oxytoca, Galleria mellonella, co-infection, transcriptome, proteome, chronic wound |
Contact List
Dörte Becher |
contact affiliation | University of Greifswald Center of Functional Genomics of Microbes Institute of Microbiology Department of Microbial Proteomics Felix-Hausdorff-Str.8 17489 Greifswald |
contact email | dbecher@uni-greifswald.de |
lab head | |
Sandra Maass |
contact affiliation | University of Greifswald, Department for Microbial Proteomics |
contact email | sandra.maass@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017208
- Label: PRIDE project
- Name: Pathogen-pathogen interactions in the microbiome, a way to soothe virulence?