PXD017187

PXD017187 is an original dataset announced via ProteomeXchange.

Title	OsNSUN2-mediated 5-methylcytidine mRNA modification enhances rice adaptation to high-temperature
Description	Heat stress is one of the extreme weather disasters potently decreasing cereal plant production. Recent studies have demonstrated that protein degradation and rRNA homeostasis as well as transcription factors are involved in thermoresponse in plants. However, how 5-methylcytosine (m5C) RNA modification contribute to heat stress response in plant remains largely unknown. Herein, we identified OsNSUN2 as the mRNA m5C methyltransferase in rice. The osnsun2 mutants specifically displayed severe phenotypes of temperature- and light-dependent lesion mimic and heat stress hypersensitivity. Heat stress enhanced mRNAs m5C methylation involving photosynthesis and detoxification systems, such as β-OsLCY, OsHO2, OsPAL1, and OsGLYI4, in an OsNSUN2-dependent manner, which then increased the protein synthesis. Consistently, the photosystem of the osnsun2 mutants was vulnerable to high ambient temperature and failed to be repaired under tolerable heat stress. Furthermore, OsNSUN2 mutation reduced photosynthesis efficiency and accumulated excessive reactive oxygen species upon heat treatment. Our findings demonstrate an important mechanism of mRNA m5C-dependent heat acclimation in rice.
HostingRepository	PRIDE
AnnounceDate	2020-04-15
AnnouncementXML	Submission_2020-04-15_02:52:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunchun GAO
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-20 00:21:14	ID requested
⏵ 1	2020-04-15 02:52:27	announced

Tang Y, Gao CC, Gao Y, Yang Y, Shi B, Yu JL, Lyu C, Sun BF, Wang HL, Xu Y, Yang YG, Chong K, OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell, 53(3):272-286.e7(2020) [pubmed]

curator keyword: Biological

submitter keyword: NSUN, rice,Oryza Sativa, heat stress, m5C methylation, RNA modification, methyltransferase, photosynthesis, ROS

Kang Chong
contact affiliation	Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
contact email	chongk@ibcas.ac.cn
lab head
Chunchun GAO
contact affiliation	Beijing Institute of Genomics
contact email	gaochunchun@big.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017187
     1. Label: PRIDE project
     2. Name: OsNSUN2-mediated 5-methylcytidine mRNA modification enhances rice adaptation to high-temperature