PXD017163

PXD017163 is an original dataset announced via ProteomeXchange.

Title	Organelle remodeling across infection time: Cytomegalovirus-host protein interactions viewed through the lens of Inter-ViSTA
Description	Nearly all biological processes rely on the finely-tuned coordination of protein interactions across cellular space and time. Accordingly, generating protein interactome datasets has become routine in biological studies, yet effectively interpreting these datasets remains computationally challenging. Here, we describe the development of Inter-ViSTA, a computational web platform for protein Interaction Visualization in Space and Time Analysis. Inter-ViSTA enables users to quickly build animated interaction networks by automatically synthesizing information on protein abundances, functional annotations, multiprotein complexes, and subcellular localizations. We then leverage Inter-ViSTA, in conjunction with quantitative mass spectrometry and molecular virology, to define the virus-host protein interactions of the human cytomegalovirus (HCMV) anti-apoptotic protein, pUL37x1 (vMIA). We find that spatially and temporally controlled protein interactions underly pUL37x1 functions during infection, facilitating the pro-viral remodeling of both mitochondria and peroxisomes. Reciprocal isolations, microscopy, and CRISPR-based genetic tools further characterize these associations, revealing new mechanisms at the core of the pUL37x1 manipulation of mitochondrial integrity, such as interactions with the MICOS and SAMM50 complexes. Finally, we show that pUL37x1 activates the peroxisomal protein PEX11ß to regulate peroxisome fission during infection, a key metabolic aspect of virus assembly and spread.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:10:07.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Federspiel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-17 03:48:54	ID requested
1	2020-08-02 22:34:43	announced
⏵ 2	2024-10-22 05:10:09	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2020.107943;

Federspiel JD, Cook KC, Kennedy MA, Venkatesh SS, Otter CJ, Hofstadter WA, Jean Beltran PM, Cristea IM, Mitochondria and Peroxisome Remodeling across Cytomegalovirus Infection Time Viewed through the Lens of Inter-ViSTA. Cell Rep, 32(4):107943(2020) [pubmed]

submitter keyword: AP-MS, UL37,HCMV, pUL37x1, PPI

Ileana Cristea
contact affiliation	Department of Molecular Biology, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Joel Federspiel
contact affiliation	Princeton University
contact email	joel.federspiel@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD017163

PRIDE project URI

• PRIDE
  1. PXD017163
     1. Label: PRIDE project
     2. Name: Organelle remodeling across infection time: Cytomegalovirus-host protein interactions viewed through the lens of Inter-ViSTA