PXD017161

PXD017161 is an original dataset announced via ProteomeXchange.

Title	Peptide N-terminal Bead Capture and Release
Description	The field of proteomics has expanded recently with more sensitive techniques for the bulk measurement of peptides as well as single-molecule techniques. One limiting factor for these methods is the need for multiple chemical derivatizations and highly pure proteins free of contaminants. We show a solid-phase capture strategy suitable for the proteolysis, purification, and subsequent chemical modification of peptides. We use this resin on an HEK293T cell lysate and perform one-pot proteolysis, capture, and derivatization to generate a cellular proteome that identified over 8,000 proteins. We also show that this capture can be reversed in a traceless manner, such that it is amenable for single-molecule proteomics techniques. With this technique, we perform a fluorescent labeling and C-terminal differentiation on a peptide and subject it to fluorosequencing demonstrating that washing the resin is sufficient to remove excess dyes prior to single-molecule protein sequencing.
HostingRepository	PRIDE
AnnounceDate	2020-09-16
AnnouncementXML	Submission_2020-09-15_22:53:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017161
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Brendan Floyd
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-17 03:48:27	ID requested
⏵ 1	2020-09-15 22:55:10	announced

Howard CJ, Floyd BM, Bardo AM, Swaminathan J, Marcotte EM, Anslyn EV, Solid-Phase Peptide Capture and Release for Bulk and Single-Molecule Proteomics. ACS Chem Biol, 15(6):1401-1407(2020) [pubmed]

submitter keyword: Human, N-terminal Modification, LC-MS/MS

Edward Marcotte
contact affiliation	University of Texas at Austin Molecular Biosciences
contact email	edward.marcotte@gmail.com
lab head
Brendan Floyd
contact affiliation	University of Texas at Austin
contact email	bmfloyd@utexas.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017161
     1. Label: PRIDE project
     2. Name: Peptide N-terminal Bead Capture and Release