PXD017158

PXD017158 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of lung tissues in chronic obstructive pulmonary disease (COPD): disfunction of platelet and macrophage contribute to pathogenesis of COPD
Description	Chronic obstructive pulmonary disease (COPD) is a worldwide public health challenge because of its high prevalence and related disability and mortality; however, the pathogenesis of COPD remains unknown. The aim of this study was to reveal the critical proteins involved in the pathogenesis of COPD. This study used high-resolution liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), in combination with quantitative 6-plex tandem mass tag labeling, to profile protein changes in lung tissues from patients with COPD and healthy donors. Western blot analyses were used to validate the proteomic data. A total of 4976 proteins were identified and analyzed. One-hundred and seventy-three proteins were found significantly changed, with 118 proteins downregulated and 55 proteins upregulated. Gene ontology analysis and protein-protein interaction networks demonstrated that the changed proteins, especially down-regulated proteins, were involved in platelet and macrophage activation. Our results indicated that abnormal platelet activation caused by down-regulation of GP6, PF4, and THBS1 contributes to the hemostasis disorders, while dysfunction of alveolar macrophages in efferocytosis, as a result of down-regulation of CD163 and MARCO, caused the failure of resolution of inflammation, and thus cause COPD.
HostingRepository	iProX
AnnounceDate	2020-01-17
AnnouncementXML	Submission_2020-07-21_19:40:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haotian Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-17 03:22:58	ID requested
⏵ 1	2020-07-21 19:40:09	announced

Liu Y, Liu H, Li C, Ma C, Ge W, Proteome Profiling of Lung Tissues in Chronic Obstructive Pulmonary Disease (COPD): Platelet and Macrophage Dysfunction Contribute to the Pathogenesis of COPD. Int J Chron Obstruct Pulmon Dis, 15():973-980(2020) [pubmed]

submitter keyword: proteomics, platelet, macrophage, COPD, pathogenesis

Wei Ge
contact affiliation	Institute of Basic Medical Sciences Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
lab head
Haotian Liu
contact affiliation	Institute of Basic Medical Sciences Chinese Academy of Medical Sciences
contact email	17824030348@163.com
dataset submitter

iProX dataset URI