PXD017153

PXD017153 is an original dataset announced via ProteomeXchange.

Title	Ex-Vivo Equine Cartilage Explant Osteoarthritis Model - A Metabolomics and Proteomics Study
Description	Osteoarthritis (OA) is an age-related degenerative musculoskeletal disease characterised by loss of articular cartilage, synovitis, abnormal bone proliferation and subchondral bone sclerosis. The underlying pathogenesis of OA is yet to be fully elucidated with no OA specific biomarkers in clinical use. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) allow identification of the global metabolome and proteome respectively. During this study, ex-vivo equine cartilage explants (n=5) were incubated in TNF-α/IL-1β supplemented culture media for 8 days, with media removed and replaced at 2, 5 and 8 days. Acetonitrile metabolite extractions of 8 day cartilage explants and media samples at all time points underwent 1H NMR metabolic analysis with media samples also undergoing MS proteomic analysis. Within the cartilage, metabolites glucose and lysine were elevated following TNF-α/IL-1β treatment whilst adenosine, alanine, betaine, creatine, myo-inositol and uridine levels decreased. Within the culture media, four, four and six metabolites were identified as being differentially abundant between control and treatment groups for 1-2 day, 3-5 day and 6-8 day time points respectively. Culture media proteomics identified 154, 138 and 72 proteins differentially abundant, with > 2 fold change, between control and treatment groups for 1-2 day, 3-5 day and 6-8 day time points respectively. Nine potential novel OA neopeptides were elevated in treated media. This is the first study to use a multi ‘omics’ approach to simultaneously investigate the metabolomic profile of ex-vivo cartilage and metabolomic/proteomic profiles of culture media using the TNF-α/IL-1β ex-vivo OA cartilage model. This study has identified a panel of metabolites, proteins and extracellular matrix derived neopeptides which are differentially abundant during an early phase of the OA model which may provide further information on underlying disease pathogenesis, allow potential translation for clinical markers and possible novel therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2020-08-03
AnnouncementXML	Submission_2020-08-02_22:21:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017153
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Anderson
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	Oxidation; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-16 07:37:41	ID requested
⏵ 1	2020-08-02 22:21:03	announced

Anderson JR, Phelan MM, Foddy L, Clegg PD, Peffers MJ, Equine Cartilage Explant Osteoarthritis Model: A Metabolomics and Proteomics Study. J Proteome Res, 19(9):3652-3667(2020) [pubmed]

submitter keyword: Osteoarthritis, Cartilage, Metabolomics, Proteomics, Nuclear Magnetic Resonance, Mass Spectrometry

Mandy Jayne Peffers
contact affiliation	Institute of Ageing and Chronic Disease, University of Liverpool, United Kingdom
contact email	peffs@liverpool.ac.uk
lab head
James Anderson
contact affiliation	University of Liverpool
contact email	janders@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017153
     1. Label: PRIDE project
     2. Name: Ex-Vivo Equine Cartilage Explant Osteoarthritis Model - A Metabolomics and Proteomics Study