PXD017150

PXD017150 is an original dataset announced via ProteomeXchange.

Title	Differentially expressed proteins wereassociated withtwo color morphs of Asian citrus psyllid, Diaphorina citri
Description	Diaphorina citriis a vector of ‘CandidatusLiberibacter asiaticus,’(CLas), associated with citrus greening or Huanglongbing (HLB) disease in citrus. D. citriexhibits two group three different color morph variants, blueand non-blue (includes gray and yellow). Blue morphs have a greater capacity for long-distance flight while has lower efficiency in CLas transmission as compared to non-blue morphswhich influences disease epidemiology. In this study, wecompare the protein profilesof two-color morphsand evaluate the effect of CLas infection on abundance of pre-identified proteins in each color morphs. Our results showed that blue morphs have higher abundance of the immunity-associated proteins, while their abundance were upregulated dramatically in the non-blue morphcompared to blue morphin result of CLas infection. This observation proposes blue morph has higher immunity and CLas trigger the immunity in both morph while in non-blue’s response is late and non-effective.Further, challenging both color morphs byentomopathogenic fungi Beauveria bassiana, were showed significantly lower mortality in blue morph vs non-blue. Also, to test the effect of delayed response in CLas acquisition, we did CLas acquisition assay after priming immunity by feeding heat-killed Liberibacter crescens to both color-morphs. The obtained results were showed that priming immunity in non-blue morph can significantly decrease CLas acquisition as close as the level of CLas acquisition in blue morph. Taking all these in account propose that higher immunity in blue morph suppress CLas acquisition in a way that CLas acts as a pathogenand costly forACP. While in non-blue ACP, CLas triggers the immunity but the delayed immunity is not effective to block CLas from acquisition.
HostingRepository	PRIDE
AnnounceDate	2021-04-27
AnnouncementXML	Submission_2021-04-26_21:56:58.899.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017150
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Howe
SpeciesList	scientific name: Diaphorina citri (Asian citrus psyllid); NCBI TaxID: 121845;
ModificationList	Methylthio; Deamidated; Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-16 05:49:37	ID requested
1	2021-04-26 09:22:46	announced
⏵ 2	2021-04-26 21:56:59	announced	2021-04-27: Updated project metadata.

10.1021/ACS.JPROTEOME.1C00089;

Hosseinzadeh S, Higgins SA, Ramsey J, Howe K, Griggs M, Castrillo L, Heck M, , Insect Vector of Citrus Greening Disease. J Proteome Res, 20(5):2851-2866(2021) [pubmed]

submitter keyword: Color polymorphism, Immunity, Proteomics survey

Michelle Heck
contact affiliation	USDA-ARS/Boyce Thompson Institute/Cornell University
contact email	mlc68@cornell.edu
lab head
Kevin Howe
contact affiliation	USDA-ARS
contact email	kjh46@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017150
     1. Label: PRIDE project
     2. Name: Differentially expressed proteins wereassociated withtwo color morphs of Asian citrus psyllid, Diaphorina citri