PXD017148

PXD017148 is an original dataset announced via ProteomeXchange.

Title	Classically-activated macrophages rely on glucose-derived glycerol and external fatty acid for TAG synthesis
Description	Altered lipid metabolism in macrophages is associated with various important inflammatory conditions. Although lipid metabolism is an important target for therapeutic intervention, the metabolic requirement involved in lipid accumulation during pro-inflammatory activation of macrophages remains incompletely characterized. We show here that macrophage activation with IFN?????? results in increased aerobic glycolysis, iNOS-dependent inhibition of respiration, and accumulation of triacylglycerol. Surprisingly, metabolite tracing with 13C-labeled glucose revealed that the glucose contributed to the glycerol groups in triacylglycerol (TAG), rather than to de novo synthesis of fatty acids. This is in stark contrast to the otherwise similar metabolism of cancer cells, and previous results obtained in activated macrophages and dendritic cells. Our results establish a novel metabolic pathway whereby glucose provides glycerol to the headgroup of TAG during classical macrophage activation.
HostingRepository	PRIDE
AnnounceDate	2020-02-27
AnnouncementXML	Submission_2020-02-27_05:03:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017148
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-16 04:02:49	ID requested
⏵ 1	2020-02-27 05:03:16	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Macrophages, Infection, TAG

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017148
     1. Label: PRIDE project
     2. Name: Classically-activated macrophages rely on glucose-derived glycerol and external fatty acid for TAG synthesis