PXD017134

PXD017134 is an original dataset announced via ProteomeXchange.

Title	A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
Description	Cells have the ability to sense, respond and adapt to environmental fluctuations. Stress causes a massive reorganization of the transcriptional program. Many examples of histone post-translational modifications (PTMs) have been associated with transcriptional activation or repression under steady-state growth conditions. Comparatively less is known about the role of histone PTMs in the cellular adaptive response to stress. Here, we performed high-throughput genetic screenings that provide a novel global map of the histone residues required for transcriptional reprogramming in response to heat and osmotic stress. Of note, we observed that the histone residues needed depend on the type of gene and/or stress, thereby suggesting a “personalized”, rather than general, subset of histone requirements for each chromatin context. In addition, we identified a number of new residues that unexpectedly serve to regulate transcription. As a proof of concept, we characterized the function of the histone residues H4-S47 and H4-T30 in response to osmotic and heat stress, respectively. Our results uncover novel roles for the kinases Cla4 and Ste20, yeast homologs of the mammalian PAK2 family, which phosphorylate H4-S47 and for the kinase Ste11 that targets H4-T30. This study provides new insights into the role of histone residues in transcriptional regulation under stress conditions.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:20:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clement Potel
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-16 01:37:59	ID requested
⏵ 1	2020-05-26 16:20:20	announced

Vi, é, itez C, Mart, í, nez-Cebri, á, n G, Sol, é C, B, ö, ttcher R, Potel CM, Savitski MM, Onnebo S, Fabregat M, Shilatifard A, Posas F, de Nadal E, A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress. Nucleic Acids Res, 48(7):3455-3475(2020) [pubmed]

curator keyword: Biological

submitter keyword: phosphoproteomics, histone, PTMs

Eulàlia de Nadal
contact affiliation	Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain
contact email	eulalia.nadal@irbbarcelona.org
lab head
Clement Potel
contact affiliation	EMBL
contact email	clement.potel@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD017134

PRIDE project URI

• PRIDE
  1. PXD017134
     1. Label: PRIDE project
     2. Name: A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress