PXD017121

PXD017121 is an original dataset announced via ProteomeXchange.

Title	Global Profiling of the Lysine Crotonylome in Different Pluripotent States
Description	Understanding the mechanisms of pluripotency maintenance and exit are important for developmental biology and regenerative medicine. However, studies of pluripotency and post-translational modifications of proteins are scarce. To systematically profile protein crotonylation in mouse pluripotent stem cells (PSCs) in different culture conditions, we used affinity purification of crotonylated peptides, TMT labeling, and LC-MS/MS. Our study included PSCs in ground, metastable, and primed state, as well as metastable PSCs undergoing early pluripotency exit. We have successfully identified 8,102 crotonylation sites in 2,578 proteins, among which 3,628 sites in 1,426 proteins were with high-confidence. These high-confidence crotonylated proteins are enriched for factors involved in functions related to pluripotency such as RNA biogenesis, central carbon metabolism, and proteasome function. Our atlas of protein crotonylation will be valuable for further studies of pluripotency regulation and may also provide insights into the role of crotonylation in other cell fate transitions.
HostingRepository	PRIDE
AnnounceDate	2021-02-26
AnnouncementXML	Submission_2021-02-25_22:42:11.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuan Lv
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-15 03:49:55	ID requested
⏵ 1	2021-02-25 22:42:11	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Metabolism, Post-translational modifications, Pluripotency, RNA-binding proteins, Proteasome

Miguel A Esteban
contact affiliation	Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
contact email	miguel@gibh.ac.cn
lab head
Yuan Lv
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	lv_yuan@gibh.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017121
     1. Label: PRIDE project
     2. Name: Global Profiling of the Lysine Crotonylome in Different Pluripotent States