PXD017109 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of cGAS acetylation and phosphorylation |
Description | DNA derived from the genetic material of pathogens or from cellular DNA damage provides a molecular pattern that can be sensed by pattern-recognition receptors of the mammalian innate immune system. In recent years, the cyclic GMP-AMP synthase (cGAS) protein has been characterized as a primary cytosolic DNA sensor during infection with bacteria, DNA viruses, or retroviruses. While the role of cGAS in downstream immune signaling through STING-TBK1-IRF3 proteins is well-defined, regulatory mechanisms of cGAS activity, such as through post-translational modifications (PTMs), are still an active area of research. Here, we report a comprehensive characterization of cGAS phosphorylations and acetylations in three different cell types. Data-dependent proteomic analyses of immunoaffinity purified cGAS was performed in HEK293T cells under control and DNA-challenged conditions (N = 3 each) and human fibroblasts (HFF) cells under control and HSV-1 infected conditions (N = 4 each) to generate candidate cGAS PTM sites. Using parallel reaction monitoring, a total of 11 PTMs (4 phosphorylations and 7 acetylations) were validated in HEK293T, HFF, and THP-1 cells. Of these, 3 phosphorylations and 5 acetylations have not been previously identified. The functions of these modifications were by generating a series of mutants and measuring cGAS-dependent apoptotic and immune signaling activities. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-11 |
AnnouncementXML | Submission_2020-05-10_22:21:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | T Greco |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-15 02:28:57 | ID requested | |
⏵ 1 | 2020-05-10 22:21:50 | announced | |
Publication List
Song B, Greco TM, Lum KK, Taber CE, Cristea IM, The DNA Sensor cGAS is Decorated by Acetylation and Phosphorylation Modifications in the Context of Immune Signaling. Mol Cell Proteomics, 19(7):1193-1208(2020) [pubmed] |
Keyword List
submitter keyword: Human, immune response, herpesvirus, PTM, affinity purification |
Contact List
Ileana M Cristea |
contact affiliation | Department of Molecular Biology, Princeton University, USA |
contact email | icristea@princeton.edu |
lab head | |
T Greco |
contact affiliation | Molecular Biology |
contact email | tgreco@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017109
- Label: PRIDE project
- Name: Identification of cGAS acetylation and phosphorylation