PXD017103 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hypoxia response in barley - proteomics analysis |
Description | Hypoxia stress (<1% oxygen) followed by reoxygenation, is accompanied by formation of reactive oxygen species (ROS) and nitric oxide (NO), which act as signaling molecules, but also cause nitro-oxidative stress. The NO concentration is regulated by phytoglobins (formerly hemoglobins) in the NO cycle. To understand how changes in phytoglobin expression affect plant metabolism under hypoxia, we studied the proteome and metabolome of barley (Hordeum vulgare L., cv. Golden Promise) plants without (WT) or with (HO) overexpression of phytoglobins. WT plants were more susceptible to hypoxia than HO. The chlorophyll a+b content was lowered by 50% and biomass by 30% in WT24, compared to WT, while HO plants were unaffected. We observe increase in ROS production during hypoxia treatment in WT seedlings had that was not observed in HO seedlings. But most importantly we identified and quantified 9694 proteins out of which 1107 changed significantly in abundance in at least one of the four comparisons – HO/WT andHO24/WT24) (giving genotype differences), WT24/WT and HO24/HO giving treatment differences. The main proteome changes between genotypes shown that many proteins were downregulated in HO24/HO but not in WT24/WT, such as ion transporters, Ca2+ signal transduction, and proteins related to protein degradation. We also identified and quantified 1470 metabolites, and the abundance of >500 changed significantly. Hypoxia had a much larger overall effect on the metabolome than overexpression of phytoglobin. The metabolites that have the biggest influence on the differences between treatments were selected for further analysis, among them many amino acids, putrescine and metabolites taking part in glycolysis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:59:51.276.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adelina Rogowska-Wrzesinska |
SpeciesList | scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-15 02:28:01 | ID requested | |
1 | 2020-03-02 00:01:19 | announced | |
⏵ 2 | 2024-10-22 04:59:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/ijms21041546; |
Andrzejczak OA, Havelund JF, Wang WQ, Kovalchuk S, Hagensen CE, Hasler-Sheetal H, Jensen ON, Rogowska-Wrzesinska A, M, ø, ller IM, Hebelstrup KH, L.) with and without Phytoglobin Priming. Int J Mol Sci, 21(4):(2020) [pubmed] |
Keyword List
submitter keyword: Barley, hypoxia, LC-MS/MS |
Contact List
Adelina Rogowska-Wrzesinska |
contact affiliation | Department of Biochemistry & Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark |
contact email | adelinar@bmb.sdu.dk |
lab head | |
Adelina Rogowska-Wrzesinska |
contact affiliation | University of Southern Denmark |
contact email | adelinar@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017103
- Label: PRIDE project
- Name: Hypoxia response in barley - proteomics analysis