PXD017100

PXD017100 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The absolute abundance of various metabolic enzymes measured by an iMPAQT method in young and nutlin3a-induced senescent (n-Sen) HCA2 cells.
Description	For senescence induction, HCA2 humn skin fibroblasts were synchronized at G2 phase by treatment with 9 uM of RO3306 (Roche) for 24 hrs, treated with 9 uM of RO3306 and 5 uM of Nutlin3a (Sigma-Aldrich) for 8 hrs, and then with 5 uM of nutlin3a for 18 hrs. Cells were then treated with 100 nM of BI-2536 for 9 days to eliminate proliferating cells, and cultured in normal media for 9 days. The senescent and non-senescent (young) cells were collected, washed with PBS, snap-frozen, and analyzed with a iMPAQT method.
HostingRepository	jPOST
AnnounceDate	2021-01-15
AnnouncementXML	Submission_2022-09-18_03:31:11.967.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yoshikazu Johmura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q TRAP

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-14 23:49:00	ID requested
1	2021-01-14 07:00:04	announced
2	2021-02-04 17:29:54	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:31:12	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: human skin fibroblasts, senescence, iMPAQT

submitter keyword: human skin fibroblasts, senescence, iMPAQT

Contact List

Makoto Nakanishi
lab head
Yoshikazu Johmura
contact affiliation	Institute of Medical Science, University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000730/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000730/