PXD017099
PXD017099 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomic analysis of the brain and colon in three rat models of irritable bowel syndrome |
Description | Background: Irritable bowel syndrome (IBS) has been gradually recognized as a disorder of the brain-gut interaction, but the molecular changes in the brain and colon that occur in disease development remain poorly understood. We employed proteomic analysis to identify differentially expressed proteins in both the brain and colon of three IBS models. Methods: To explore the relevant protein abundance changes in the brain and colon, isobaric tags for relative and absolute quantitation (iTRAQ), liquid chromatography and tandem mass spectrometry (LC-MS) and Western blotting methods were used in three IBS models, including maternal separation (MS, group B), chronic wrap restraint stress (CWRS, group C) and a combination of MS and CWRS (group D). Results: We identified 153, 280, and 239 proteins that were common and differentially expressed in the two tissue types of groups B, C and D, respectively; 43 differentially expressed proteins showed the same expression changes among the three groups, including 25 proteins upregulated in the colon and downregulated in the brain, 7 proteins downregulated in the colon and upregulated in the brain, and 3 proteins upregulated and 8 downregulated in both tissues. Gene ontology analysis showed that the differentially expressed proteins were mainly associated with cellular assembly and organization and cellular function and maintenance. Protein interaction network and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions: Taken together, the data presented represent a comprehensive and quantitative proteomic analysis of the brain and colon in IBS models, providing new evidence of an abnormal brain-gut interaction in IBS. These data may be useful for further investigation of potential targets in the diagnosis and treatment of IBS. |
HostingRepository | iProX |
AnnounceDate | 2020-01-15 |
AnnouncementXML | Submission_2020-11-25_22:50:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hong Xue |
SpeciesList | scientific name: Rattus rattus; NCBI TaxID: 10117; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-01-14 23:06:54 | ID requested | |
1 | 2020-01-14 23:08:18 | announced | |
⏵ 2 | 2020-11-25 22:50:03 | announced | 2020-11-26: Update publication information |
Publication List
Zhang B, Xue H, Wang W, Chen T, Su M, Kang N, Yang J, Bian Z, Wang F, Tang X, Comparative proteomic analysis of the brain and colon in three rat models of irritable bowel syndrome. Proteome Sci, 18():1(2020) [pubmed] |
Keyword List
submitter keyword: irritable bowel syndrome models, proteomics, brain, colon |
Contact List
Xudong Tang | |
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contact affiliation | China Academy of Chinese Medical Sciences |
contact email | txdly@sina.com.cn |
lab head | |
Hong Xue | |
contact affiliation | Xiyuan Hospital, China Academy of Chinese Medical Sciences |
contact email | xuehong03@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |