PXD017090 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Antibacterial properties and mechanism of action for serum obtained from the Chinese alligator (Alligator sinensis) |
Description | Chinese alligator (Alligator sinensis) is one of the rarest endangered reptiles found in China and possesses strong immune potential. This study tested the antibacterial ability of Chinese alligator serum (CAS) against Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and analyzed the underlying mechanisms. Results showed that the CAS had a marked antibacterial effect on K. pneumoniae, E. coli, and P. aeruginosa. However, S. aureus was only mildly affected, and this effect disappeared when incubated with Protease K. The serum proteome revealed that the antibacterial ability of CAS was produced by interactions between various proteins and that the complement proteins played a major antibacterial role. Furthermore, the prediction of the structure and function of complement component 3 revealed eight potential protein binding sites and one nucleic acid binding site that were likely related to the broad-spectrum antibacterial ability of this serum. This study provided evidence that CAS elicits significant antibacterial effects against some pathogens and provides the basis for further development of novel antibacterial drugs. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:12:32.109.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sheng-Guo Fang |
SpeciesList | scientific name: Alligator sinensis; NCBI TaxID: 38654; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-14 06:19:12 | ID requested | |
1 | 2023-03-10 13:19:33 | announced | |
⏵ 2 | 2023-11-14 08:12:37 | announced | 2023-11-14: Updated project metadata. |
Publication List
Hu MY, Chen YW, Chai ZF, Wang YZ, Lin JQ, Fang SG, Antibacterial Properties and Potential Mechanism of Serum from Chinese Alligator. Microorganisms, 10(11):(2022) [pubmed] |
Keyword List
submitter keyword: Protein, Antibacterial, Alligator sinensis, Complement system,Serum |
Contact List
Sheng-Guo Fang |
contact affiliation | MOE Key Laboratory of Biosystems Homeostasis & Protection, State Conservation Centre for Gene Resources of Endangered Wildlife, College of Life Sciences, Zhejiang University, Hangzhou 310058, P. R. China |
contact email | sgfanglab@zju.edu.cn |
lab head | |
Sheng-Guo Fang |
contact affiliation | Zhejiang University |
contact email | sgfanglab@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017090
- Label: PRIDE project
- Name: Antibacterial properties and mechanism of action for serum obtained from the Chinese alligator (Alligator sinensis)