PXD017090

PXD017090 is an original dataset announced via ProteomeXchange.

Title	Antibacterial properties and mechanism of action for serum obtained from the Chinese alligator (Alligator sinensis)
Description	Chinese alligator (Alligator sinensis) is one of the rarest endangered reptiles found in China and possesses strong immune potential. This study tested the antibacterial ability of Chinese alligator serum (CAS) against Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and analyzed the underlying mechanisms. Results showed that the CAS had a marked antibacterial effect on K. pneumoniae, E. coli, and P. aeruginosa. However, S. aureus was only mildly affected, and this effect disappeared when incubated with Protease K. The serum proteome revealed that the antibacterial ability of CAS was produced by interactions between various proteins and that the complement proteins played a major antibacterial role. Furthermore, the prediction of the structure and function of complement component 3 revealed eight potential protein binding sites and one nucleic acid binding site that were likely related to the broad-spectrum antibacterial ability of this serum. This study provided evidence that CAS elicits significant antibacterial effects against some pathogens and provides the basis for further development of novel antibacterial drugs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:12:32.109.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sheng-Guo Fang
SpeciesList	scientific name: Alligator sinensis; NCBI TaxID: 38654;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-14 06:19:12	ID requested
1	2023-03-10 13:19:33	announced
⏵ 2	2023-11-14 08:12:37	announced	2023-11-14: Updated project metadata.

Hu MY, Chen YW, Chai ZF, Wang YZ, Lin JQ, Fang SG, Antibacterial Properties and Potential Mechanism of Serum from Chinese Alligator. Microorganisms, 10(11):(2022) [pubmed]

submitter keyword: Protein, Antibacterial, Alligator sinensis, Complement system,Serum

Sheng-Guo Fang
contact affiliation	MOE Key Laboratory of Biosystems Homeostasis & Protection, State Conservation Centre for Gene Resources of Endangered Wildlife, College of Life Sciences, Zhejiang University, Hangzhou 310058, P. R. China
contact email	sgfanglab@zju.edu.cn
lab head
Sheng-Guo Fang
contact affiliation	Zhejiang University
contact email	sgfanglab@zju.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017090
     1. Label: PRIDE project
     2. Name: Antibacterial properties and mechanism of action for serum obtained from the Chinese alligator (Alligator sinensis)