PXD017089

PXD017089 is an original dataset announced via ProteomeXchange.

Title	Integrative genomic and proteomic studies of Leishmania donovani strains revealed genetic features associated with drug resistance
Description	Leishmaniasis is a neglected tropical disease affecting millions of people worldwide. Emerging drug resistance of Leishmania species poses threaten to the effective control and elimination of this neglected tropical disease. Here we conducted whole genome resequencing, proteome profiling, and comparative analyses of a drug-resistant clinical isolate and two drug-susceptible strains of Leishmania donovani to explore genetic features that might contribute to the establishment of drug resistance in this parasite. By comparative genomic analysis, exclusive variations were identified in the drug-resistant isolate of L. donovani, including 86 copy number variations, 271 frameshift mutations in protein-coding genes and two site mutations in non-coding genes. Comparative proteomic analysis indicated significant differences in protein expression between resistant and susceptible strains of L. donovani, including 69 exclusive detected molecules and 84 consistent down-/up-regulations in the former. Integrating the genomic mutations and proteomic specificities linked nine of the genomic mutations (gene duplication, insertion and deletion) to significantly altered protein expression changes in the drug-resistant clinical isolate. These genetic features were inferred to be associated with nucleotide-binding and fatty acid metabolism (biosynthesis and degradation), which might contribute to fitness-gains allowing for the drug-resistant phenotype of L. donovani. This comparative and integrative work provided deep insights into the molecular basis underlying resistance establishment, suggesting new aspects to be investigated for novel intervention strategies against L. donovani and related species.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:59.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiao Li
SpeciesList	scientific name: Leishmania ; NCBI TaxID: 38568;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-14 02:16:43	ID requested
1	2021-09-08 17:16:10	announced
⏵ 2	2024-10-22 05:27:07	announced	2024-10-22: Updated project metadata.

Zheng Z, Chen J, Ma G, Satoskar AR, Li J, Integrative genomic, proteomic and phenotypic studies of Leishmania donovani strains revealed genetic features associated with virulence and antimony-resistance. Parasit Vectors, 13(1):510(2020) [pubmed]

10.1186/s13071-020-04397-4;

submitter keyword: Genomic

proteomic

Leishmania donovani

drug resistance

Jiao Li
contact affiliation	Department of Pathogenic Biology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China
contact email	joyleeql2019@163.com
lab head
Jiao Li
contact affiliation	West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China
contact email	joyleeql2019@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017089
     1. Label: PRIDE project
     2. Name: Integrative genomic and proteomic studies of Leishmania donovani strains revealed genetic features associated with drug resistance