PXD017086

PXD017086 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic characterization of probiotics
Description	The manufacturing processes of commercial probiotic strains may be affected in different ways in the attempt to optimize yield, costs, functionality, or stability, influencing gene expression, protein patterns, or metabolic output. Aim of this work was to compare different samples of a high concentration (450 billion bacteria) multispecies (8 strains) formulation produced at two different manufacturing sites, USA (US) and Italy (IT), by applying functional proteomics analyses. Several protein-profile differences were detected between IT- and US-made products, with Lactobacillus paracasei, Streptococcus thermophilus, and Bifidobacteria being the main affected probiotics/microorganisms. Integration of omics platforms with in vivo analysis emerged as a powerful tool to assess manufacturing procedures.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:59:04.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benedetta Mattei
SpeciesList	scientific name: Lactobacillus acidophilus NCFM; NCBI TaxID: 272621; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-14 01:31:56	ID requested
1	2020-01-22 05:00:18	announced
2	2020-02-17 03:21:37	announced	2020-02-17: Updated publication reference for PubMed record(s): 31963736.
⏵ 3	2024-10-22 04:59:07	announced	2024-10-22: Updated project metadata.

Publication List

10.3390/biom10010157;
Bianchi L, Laghi L, Correani V, Schifano E, Landi C, Uccelletti D, Mattei B, A Combined Proteomics, Metabolomics and In Vivo Analysis Approach for the Characterization of Probiotics in Large-Scale Production. Biomolecules, 10(1):(2020) [pubmed]

10.3390/biom10010157;

Bianchi L, Laghi L, Correani V, Schifano E, Landi C, Uccelletti D, Mattei B, A Combined Proteomics, Metabolomics and In Vivo Analysis Approach for the Characterization of Probiotics in Large-Scale Production. Biomolecules, 10(1):(2020) [pubmed]

Keyword List

submitter keyword: probiotic quality assessment, functional proteomics

submitter keyword: probiotic quality assessment, functional proteomics

Contact List

Benedetta Mattei
contact affiliation	Department of Life, Health and Environmental Sciences
contact email	mariabenedetta.mattei@univaq.it
lab head
Benedetta Mattei
contact affiliation	University of L'Aquila
contact email	mariabenedetta.mattei@univaq.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017086
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017086

PRIDE project URI

Repository Record List

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