PXD017078

PXD017078 is an original dataset announced via ProteomeXchange.

Title	Nanopillar surface mediates antibacterial effects via cell impedance, penetration and induction of oxidative stress
Description	Abstract. The adhesion of bacteria to medical implants and formation of biofilms is a growing healthcare problem that accounts for a significant proportion of hospital-acquired infections globally. Insects, including cicada and dragonfly, have evolved nanoprotrusions-protruding arrays on their wings that rupture bacteria on contact. This has inspired the design of antibacterial implant surfaces with insect wing mimetic nanopillarsarrays . Here we characterise the physiological and morphological responses of bacteria to dragonfly wing mimetic nanowirenanopillars arrays, with the aim of determining the mechanistic basis for antibacterial activity. Dragonfly wing mimetic nanowirenanopillars arrays induce deformation and penetration of Gram-positive and Gram-negative bacterial envelopes, but do not rupture or lyse bacteria. NanowireNanopillars also have capacity to impede bacterial division and trigger reactive oxygen species production oxidative stress responses, leading to the increased abundance of oxidative stress proteins. NanowireNanopillar-induced oxidative stress represents a novel antibacterial mechanism of biomimetic nanotopographies .arrays Better understanding of this could prove invaluable for enhancing the bactericidal performance of nanotextured materials for next generation antibacterial medical implants
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:50.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joshua Jenkins
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-13 03:50:58	ID requested
1	2020-05-26 16:05:36	announced
⏵ 2	2024-10-22 05:05:58	announced	2024-10-22: Updated project metadata.

10.1038/s41467-020-15471-x;

Jenkins J, Mantell J, Neal C, Gholinia A, Verkade P, Nobbs AH, Su B, Antibacterial effects of nanopillar surfaces are mediated by cell impedance, penetration and induction of oxidative stress. Nat Commun, 11(1):1626(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Escherichia coli, Nanopillars,Staphylococcus aureus

Bo Su
contact affiliation	Bristol Dental School, University of Bristol
contact email	b.su@bristol.ac.uk
lab head
Joshua Jenkins
contact affiliation	University of British Columbia
contact email	joshua.j.jenkins@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017078
     1. Label: PRIDE project
     2. Name: Nanopillar surface mediates antibacterial effects via cell impedance, penetration and induction of oxidative stress