PXD017076

PXD017076 is an original dataset announced via ProteomeXchange.

Title	Stress enhances secretory autophagy increasing extracellular Bdnf maturation
Description	Stress response is a fundamental mechanism to maintain a healthy homeostasis and its disruption is implicated in several psychiatric disorders. Autophagy is one of the mechanisms regulating homeostasis, and it is known to be activated by cellular stressors such as starvation. In a previous study, we revealed that glucocorticoid (GC)-mediated stress also activates macroautophagy via the stress-responsive co-chaperone FK506-binding protein 51 (FKBP51). In this study we reveal that GC-mediated stress enhances secretory autophagy, a recently described Atg-dependent secretory pathway, via FKBP51. Strikingly we detected the matrix metallopeptidase 9 (Mmp9) as one of the stress-induced secreted proteins. Furthermore we found that enhanced Mmp9 secretion increases both in vitro and in vivo the cleavage of pro-brain derived neurotrophic factor (proBdnf) to its mature form (mBdnf), a key protein in neuroplasticity. This unravelled mechanism can represent a link between stress and the development of psychiatric disorders.
HostingRepository	PRIDE
AnnounceDate	2021-08-05
AnnouncementXML	Submission_2021-08-05_02:46:52.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Svenja Wiechmann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-12 23:59:07	ID requested
⏵ 1	2021-08-05 02:46:53	announced

Martinelli S, Anderzhanova EA, Bajaj T, Wiechmann S, Dethloff F, Weckmann K, Heinz DE, Ebert T, Hartmann J, Geiger TM, D, ö, ngi M, Hafner K, P, ö, hlmann ML, Jollans L, Philipsen A, Schmidt SV, Schmidt U, Maccarrone G, Stein V, Hausch F, Turck CW, Schmidt MV, Gellner AK, Kuster B, Gassen NC, Stress-primed secretory autophagy promotes extracellular BDNF maturation by enhancing MMP9 secretion. Nat Commun, 12(1):4643(2021) [pubmed]

curator keyword: Biological

submitter keyword: Secretome, secretory autophagy, stress

Bernhard Kuster
contact affiliation	Technical University of Munich, Chair of Proteomics and Bioanalytics
contact email	kuster@tum.de
lab head
Svenja Wiechmann
contact affiliation	TU Munich, Proteomics and Bioanalytics
contact email	svenja.wiechmann@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD017076

PRIDE project URI

• PRIDE
  1. PXD017076
     1. Label: PRIDE project
     2. Name: Stress enhances secretory autophagy increasing extracellular Bdnf maturation