PXD017075

PXD017075 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Facile sample preparation for quantitative single-cell proteomics
Description	Large numbers of cells are generally required for quantitative global proteome profiling due to the significant surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible for rare cell populations, such as circulating tumor cells (CTCs) and early metastatic cells. Herein we report a facile mass spectrometry (MS)-based single-cell proteomics method that capitalizes on a MS-compatible nonionic surfactant, n-Dodecyl-β-D-maltoside, for greatly reducing the surface adsorption losses by ~20-fold for effective single-tube processing of single cells, thus significantly improving detection sensitivity for single-cell proteomic analysis. With standard MS platforms, the method allows for the first time precise, label-free, reliable quantification of hundreds of proteins from single human cells in a simple, convenient manner. When applied to a patient CTC-derived xenograft (PCDX) model, the method can reveal distinct protein signatures between primary tumor cells and early metastases to the lungs at the single-cell resolution. The approach paves the way for routine, precise quantitative single-cell proteomic analysis.
HostingRepository	jPOST
AnnounceDate	2021-06-07
AnnouncementXML	Submission_2022-09-18_03:11:59.227.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tujin Shi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxymethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive; LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-12 19:06:00	ID requested
1	2021-06-06 08:00:05	announced
⏵ 2	2022-09-18 03:11:59	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Patient CTC-derived xenograft (PCDX), single cells, single-cell proteomics, surfactant-assisted single-tube processing coupled with MS (SST-MS), nonionic surfactant

submitter keyword: Patient CTC-derived xenograft (PCDX), single cells, single-cell proteomics, surfactant-assisted single-tube processing coupled with MS (SST-MS), nonionic surfactant

Contact List

Tujin Shi
lab head
Tujin Shi
contact affiliation	Pacific Northwest National Laboratory
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000733/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000733/