PXD017073

PXD017073 is an original dataset announced via ProteomeXchange.

Title	Metabolomic analysis identifies metabolites associated with systemic acquired resistance in Arabidopsis
Description	Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected distal leaves following an initial localized infection. However, little information is available on the molecular biological basis of SAR especially in uninfected distal leaves. Here, we used two SAR-induced bacteria to induce SAR in Arabidopsis, a metabolomics approach based on ultra-high-performance liquid chromatography (UPLC) and mass spectrometry (MS) technique was used to identify SAR-related metabolites in SAR-induced bacteria infected local leaves and distal leaves of locally SAR-induced bacteria infected plants. Multiple statistical analyses were used to identify differentially expressed metabolites (DEMs). The result showed primary metabolism and secondary metabolism were significantly altered in local leaves and distal leaves, including phenolic compounds, amino acids, nucleotides, organic acids and many other metabolites. Furthermore, the content of amino acids, phenolic compounds, coenzymes and its derivatives increased in distal leaves, suggesting these metabolites contributed to the establishment of SAR. In addition, 2’-hydroxy-4,4’,6’-trimethoxychalcone, phenylalanine and ethyl docosahexaenoate were identified as potential components which may play important role both in basic resistance and SAR. This study provided a reference for understanding metabolic mechanism associated with SAR in Arabidopsis, which will be useful for further investigation of the molecular basis of SAR.
HostingRepository	iProX
AnnounceDate	2020-05-05
AnnouncementXML	Submission_2020-11-12_20:31:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Zhou
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-11 18:25:43	ID requested
1	2020-05-04 19:32:51	announced
⏵ 2	2020-11-12 20:31:26	announced	2020-11-13: Update publication information.

Gao H, Zhou Q, Yang L, Zhang K, Ma Y, Xu ZQ, Metabolomics analysis identifies metabolites associated with systemic acquired resistance in Arabidopsis. PeerJ, 8():e10047(2020) [pubmed]

submitter keyword: Metabolomics, Systemic acquired resistance, Arabidopsis, Amino acids, Phenolic compounds

Ziqin Xu
contact affiliation	Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University
contact email	ziqinxu@nwu.edu.cn
lab head
Qian Zhou
contact affiliation	Shanghai Omicsspace Biotech Co., Ltd.
contact email	zhouqian@omicsspace.com
dataset submitter

iProX dataset URI