PXD017068

PXD017068 is an original dataset announced via ProteomeXchange.

Title	UTP18 as an oncogene promotes colorectal adenoma to carcinoma progression
Description	Colorectal cancer often develops slowly from adenoma. In this study, we aimed to identify the oncogenes involved in the progression of colorectal adenoma to carcinoma by Tandem Mass Tag (TMT)-based quantitative proteomics. Protein expression changes were compared by TMT-based quantitative mass spectrometry in seven paired samples of normal mucosa, adenoma and carcinoma. Moreover, a bioinformatics analysis of differential protein expression was performed to screen for oncogenes. Effects of oncogene knockdown on cell viability, proliferation, migration and invasion were analyzed in colorectal cancer cell lines. Effects of oncogene overexpression on cell viability and proliferation were analyzed in adenoma organoids. The protein UTP18 was consistently upregulated in the normal-adenoma-carcinoma sequence. UTP18 downregulation was shown to inhibit colorectal cancer cell viability, proliferation, migration and invasion. UTP18 overexpression promoted adenoma organoid viability and proliferation. UTP18 facilitated the expression of MYC and impaired expression of the tumor-suppressor p21. Our data revealed the importance of UTP18 as an effector in the progression of adenoma to carcinoma. Thus, UTP18 is implicated as a highly specific biomarker for early diagnosis of progressive adenomas.
HostingRepository	iProX
AnnounceDate	2020-06-01
AnnouncementXML	Submission_2023-03-01_19:06:51.452.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dan Luo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-10 06:56:16	ID requested
1	2020-06-01 00:53:54	announced
⏵ 2	2023-03-01 19:06:52	announced	2023-03-02: Update publication information.

Zhang Y, Li CY, Ge W, Xiao Y, Exploration of the Key Proteins in the Normal-Adenoma-Carcinoma Sequence of Colorectal Cancer Evolution Using In-Depth Quantitative Proteomics. J Oncol, 2021():5570058(2021) [pubmed]

submitter keyword: Colorectal adenoma to carcinoma progression, UTP18, Oncogene

Wei Ge
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
lab head
Dan Luo
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	luoxiaobaipumc@163.com
dataset submitter

iProX dataset URI