PXD017056

PXD017056 is an original dataset announced via ProteomeXchange.

Title	VESICULATION OF RED BLOOD CELLS IN THE BLOOD BANK: A MULTI-OMICS APPROACH TOWARDS IDENTIFICATION OF CAUSES AND CONSEQUENCES
Description	Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanism of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic and metabolomic analyses in order to obtain an integrated understanding of the mechanisms underlying microvesicle generation during storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:46.645.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vikram Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-10 03:30:52	ID requested
1	2020-04-03 00:44:39	announced
2	2020-06-12 04:27:54	announced	2020-06-12: Updated publication reference for PubMed record(s): 32244435.
⏵ 3	2024-10-22 05:01:47	announced	2024-10-22: Updated project metadata.

Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R, Bosman G, Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences. Proteomes, 8(2):(2020) [pubmed]

10.3390/proteomes8020006;

submitter keyword: Metabolomics, Blood, Lipidomics,Proteomics, RBC

Giel Bosman
contact affiliation	Department of Biochemistry (286) Radboud University Medical Centre Radboud Institute for Molecular Life Sciences P.O. Box 9101 NL-6500 HB Nijmegen The Netherlands
contact email	giel.bosman@radboudumc.nl
lab head
Vikram Sharma
contact affiliation	Plymouth University Peninsula Schools of Medicine and Dentistry
contact email	vikram.sharma@plymouth.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017056

PRIDE project URI

• PRIDE
  1. PXD017056
     1. Label: PRIDE project
     2. Name: VESICULATION OF RED BLOOD CELLS IN THE BLOOD BANK: A MULTI-OMICS APPROACH TOWARDS IDENTIFICATION OF CAUSES AND CONSEQUENCES