PXD017056 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | VESICULATION OF RED BLOOD CELLS IN THE BLOOD BANK: A MULTI-OMICS APPROACH TOWARDS IDENTIFICATION OF CAUSES AND CONSEQUENCES |
Description | Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanism of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic and metabolomic analyses in order to obtain an integrated understanding of the mechanisms underlying microvesicle generation during storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:01:46.645.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vikram Sharma |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-10 03:30:52 | ID requested | |
1 | 2020-04-03 00:44:39 | announced | |
2 | 2020-06-12 04:27:54 | announced | 2020-06-12: Updated publication reference for PubMed record(s): 32244435. |
⏵ 3 | 2024-10-22 05:01:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R, Bosman G, Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences. Proteomes, 8(2):(2020) [pubmed] |
10.3390/proteomes8020006; |
Keyword List
submitter keyword: Metabolomics, Blood, Lipidomics,Proteomics, RBC |
Contact List
Giel Bosman |
contact affiliation | Department of Biochemistry (286) Radboud University Medical Centre Radboud Institute for Molecular Life Sciences P.O. Box 9101 NL-6500 HB Nijmegen The Netherlands |
contact email | giel.bosman@radboudumc.nl |
lab head | |
Vikram Sharma |
contact affiliation | Plymouth University Peninsula Schools of Medicine and Dentistry |
contact email | vikram.sharma@plymouth.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017056
- Label: PRIDE project
- Name: VESICULATION OF RED BLOOD CELLS IN THE BLOOD BANK: A MULTI-OMICS APPROACH TOWARDS IDENTIFICATION OF CAUSES AND CONSEQUENCES