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PXD017056

PXD017056 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVESICULATION OF RED BLOOD CELLS IN THE BLOOD BANK: A MULTI-OMICS APPROACH TOWARDS IDENTIFICATION OF CAUSES AND CONSEQUENCES
DescriptionMicrovesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanism of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic and metabolomic analyses in order to obtain an integrated understanding of the mechanisms underlying microvesicle generation during storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:01:46.645.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVikram Sharma
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-10 03:30:52ID requested
12020-04-03 00:44:39announced
22020-06-12 04:27:54announced2020-06-12: Updated publication reference for PubMed record(s): 32244435.
32024-10-22 05:01:47announced2024-10-22: Updated project metadata.
Publication List
Freitas Leal JK, Lasonder E, Sharma V, Schiller J, Fanelli G, Rinalducci S, Brock R, Bosman G, Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences. Proteomes, 8(2):(2020) [pubmed]
10.3390/proteomes8020006;
Keyword List
submitter keyword: Metabolomics, Blood, Lipidomics,Proteomics, RBC
Contact List
Giel Bosman
contact affiliationDepartment of Biochemistry (286) Radboud University Medical Centre Radboud Institute for Molecular Life Sciences P.O. Box 9101 NL-6500 HB Nijmegen The Netherlands
contact emailgiel.bosman@radboudumc.nl
lab head
Vikram Sharma
contact affiliationPlymouth University Peninsula Schools of Medicine and Dentistry
contact emailvikram.sharma@plymouth.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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