PXD017055

PXD017055 is an original dataset announced via ProteomeXchange.

Title	Non-coding divergence of the human X and Y chromosomes drove male-biased expression of dosage-sensitive regulators
Description	Little is known about how Y-chromosome gene expression directly contributes to differences between XX (female) and XY (male) individuals in non-reproductive tissues. It is often said that Y-chromosome genes show low expression outside the testis. Here, we analyzed quantitative profiles of Y-chromosome gene expression across 36 human tissues from hundreds of individuals and report many instances where a Y-chromosome gene shows upregulated expression in a non-reproductive tissue. A notable example is EIF1AY, which encodes eukaryotic initiation factor 1A (EIF1A), together with its X-linked homolog EIF1AX. Evolutionary loss of a Y-linked microRNA target site enabled upregulation of EIF1AY, but not EIF1AX, in the heart. As a result, this essential translation initiation factor is nearly twice as abundant in male as in female heart tissue at the protein level. Divergence between the X and Y chromosomes in regulatory sequence can therefore lead to tissue-specific, Y-chromosome-driven sex biases in expression of critical, dosage-sensitive regulatory genes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:05.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Chick
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-10 02:59:57	ID requested
1	2021-09-08 17:06:33	announced
⏵ 2	2024-10-22 05:27:09	announced	2024-10-22: Updated project metadata.

Godfrey AK, Naqvi S, Chm, á, tal L, Chick JM, Mitchell RN, Gygi SP, Skaletsky H, Page DC, Quantitative analysis of Y-Chromosome gene expression across 36 human tissues. Genome Res, 30(6):860-873(2020) [pubmed]

10.1101/gr.261248.120;

submitter keyword: Tandem mass tags,Proteomics, Orbitrap Lumos, LC-MS/MS

Steven Gygi
contact affiliation	Cell Biology Harvard Medical School
contact email	steven_gygi@hms.harvard.edu
lab head
Joel Chick
contact affiliation	Harvard Medical School
contact email	chick.joel@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD017055

PRIDE project URI

• PRIDE
  1. PXD017055
     1. Label: PRIDE project
     2. Name: Non-coding divergence of the human X and Y chromosomes drove male-biased expression of dosage-sensitive regulators