PXD017047

PXD017047 is an original dataset announced via ProteomeXchange.

Title	Isolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves.
Description	Rotaviruses (RVs) account for severe diarrhea in children and young animals globally. In the current study, the fecal samples of diarrheic calves from a beef farm in Inner Mongolia were screened for RVA by ELISA and RT- PCR, followed by culture of three positive RVA samples in the MA-104 cell line. After 10 blind passages, cytopathic effects (CPE) appeared as detachment, granulation, and clustering of the inoculated cells. The virus isolates were identified by RT-PCR (VP6 gene RVA) and ESI-LC-MS/MS for whole protein sequencing. The protein sequences demonstrated the presence of two strains from species A rotavirus and one RVB strain; RVA/Cow-tc/ CHN/35333/2019/G6P[5] was mixed with one RVB strain (RVB/Cow-tc/CHN/35334/2019/G5P[3]) in two samples, and RVA/Cow-tc/CHN/10927/2019/G8P[7] was found in one sample. They are of genotype constellations (G6-P[5]-I2-R2-C2-M2-A3-N2-T6-E2-H3), (G8-P[7]-I5-R1-C1- M2-A1-N1-T1-E1-H1), and (G5-P[3]-I3-R5- C5-A5-N4-H5), respectively. Besides, phylogenetic analysis of the obtained sequences demonstrated viral evolution.
HostingRepository	PRIDE
AnnounceDate	2021-01-18
AnnouncementXML	Submission_2021-01-17_23:59:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gehad Elkady
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	S-pyridylethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-10 01:22:39	ID requested
⏵ 1	2021-01-17 23:59:58	announced

Elkady G, Zhu J, Peng Q, Chen M, Liu X, Chen Y, Hu C, Chen H, Guo A, Isolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves. Infect Genet Evol, 89():104715(2021) [pubmed]

curator keyword: Biological

submitter keyword: Bovine Rotavirus, BRA,BRB, Protein typing, ESl-LC- MS/MS

Aizhen Guo
contact affiliation	College of Veterinary Medicine, The state Key Laboratory of Agricultural Microbiology, Cooperative Innovation Center of Substantial Pig Production, Wuhan, 433070, China
contact email	aizhen@mail.hzau.edu.cn
lab head
Gehad Elkady
contact affiliation	College of Veterinary Medicine, HZAU
contact email	2307957696@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017047
     1. Label: PRIDE project
     2. Name: Isolation and whole protein characterization of species A and B bovine rotaviruses from Chinese calves.