PXD017044

PXD017044 is an original dataset announced via ProteomeXchange.

Title	LFQ_TurboID-STING_DMXAA_Biotin_Antibody_RAW2647
Description	The STING-null RAW264.7 cells expressing TurboID-STING were treated with DMSO or 100 μg/ml DMXAA, and 500 µM biotin was added to the medium. After 1-h incubation, the cells were lysed in 6 M guanidine-HCl containing 100 mM Tris-HCl (pH 8.0) and 2 mM DTT. The lysates were dissolved by heating and sonication, and centrifuged at 20,000 g for 15 min at 4ºC. The supernatants were recovered, followed by reduction in 5 mM DTT at room temperature for 30 min and alkylation in 27.5 mM iodoacetamide at room temperature for 30 min in the dark. Proteins were purified by methanol/chloroform precipitation and solubilized by 0.1% RapiGest SF in 50 mM triethylammonium bicarbonate. After repeated sonication and vortex, the proteins were digested with 20 µg trypsin at 37ºC overnight. The resultant peptide solutions were captured on the anti-biotin antibody-coupled beads in the presence of 1 mg/ml Pefabloc SC during 16 h of incubation at 4ºC. After washing with TBS four times, and with ultrapure water twice, the biotinylated peptides were eluted with 50 µL of 0.1% TFA in 80% acetonitrile three times. The combined eluates were evaporated, and desalted using GL-Tip SDB. The desalted eluates were further evaporated, and redissolved in 0.1% TFA.
HostingRepository	jPOST
AnnounceDate	2021-01-10
AnnouncementXML	Submission_2022-09-18_03:19:54.517.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; N6-biotinyl-L-lysine; L-methionine sulfoxide
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-09 19:22:05	ID requested
1	2021-01-09 07:00:04	announced
2	2021-02-04 16:38:05	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:19:54	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: TurboID, STING, anti-biotin antibody

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

jPOST dataset URI

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