PXD017034

PXD017034 is an original dataset announced via ProteomeXchange.

Title	Identification of the intermolecular disulfide bonds between NPGPx and ZAP70
Description	The tyrosine kinase, ζ-chain-associated protein of 70 kDa (ZAP70), is essential for T cell development and activation. However, it remains elusive whether a direct redox regulation affects ZAP70 activity upon TCR stimulation. Here, we showed that deficiency of non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx), a redox sensor, resulted in T cell hyperproliferation and elevated cytokine productions. Through proteomic approaches, ZAP70 is identified as the key interacting protein of NPGPx through disulfide bonding. Here we utilized the MS/MS spectra to identify the cysteine residues in ZAP70 for NPGPx-mediated regulation. Combined molecular approaches, our results elucidate a delicate redox mechanism that NPGPx serves as a modulator to curb ZAP70 functions in maintaining T cell homeostasis.
HostingRepository	PRIDE
AnnounceDate	2021-01-25
AnnouncementXML	Submission_2021-01-24_23:00:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pang-Hung Hsu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-09 02:26:00	ID requested
⏵ 1	2021-01-24 23:00:14	announced

Su FY, Huang SC, Wei PC, Hsu PH, Li JP, Su LW, Hsieh YL, Hu CM, Hsu JL, Yang CY, Chung CY, Shew JY, Lan JL, Sytwu HK, Lee EY, Lee WH, Redox sensor NPGPx restrains ZAP70 activity and modulates T cell homeostasis. Free Radic Biol Med, 165():368-384(2021) [pubmed]

curator keyword: Biological

submitter keyword: NPGPx, ZAP70, disulfide bond

Pang-Hung Hsu
contact affiliation	National Taiwan Ocean University
contact email	phsu@ntou.edu.tw
lab head
Pang-Hung Hsu
contact affiliation	National Taiwan Ocean University
contact email	phsu@ntou.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017034
     1. Label: PRIDE project
     2. Name: Identification of the intermolecular disulfide bonds between NPGPx and ZAP70