PXD017032

PXD017032 is an original dataset announced via ProteomeXchange.

Title	Comparative phosphoproteomic analysis of sporulated oocysts between virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns
Description	Phosphorylation is a regulatory mechanism by which intracellular apicomplexan parasites control the process of invading and modifying host cells. However, the sporulated oocysts’ phosphoprotein repertoires between virulent and avirulent strains of Toxoplasma gondii and the underlying mechanisms contributing to virulence is still a mystery. A quantitative analysis of the sporulated oocysts’ phosphoproteome profile of T. gondii virulent and avirulent strains belonging to different genotypes was conducted to reveal phosphoprotein expression patterns that contribute to the virulence of a particular phenotype. Phosphopeptides from the sporulated oocysts of the virulent strain PYS (Chinese ToxoDB#9) and the avirulent strain type II (PRU strain) were enriched by titanium dioxide (TiO2) affinity chromatography and quantified using iBT technology. A total of 10,645 unique phosphopeptides, 8,181 nonredundant phosphorylation sites and 2,792 phosphoproteins were identified. The quantitative analysis identified 4,129 differentially expressed phosphopeptides (DEPs) between sporulated oocysts of the PYS strain and PRU strain (|log1.5 fold change| > 1 and P < 0.05), including 2,485 upregulated and 1,644 downregulated phosphopeptides. Motif analysis identified 24 motifs from the upregulated phosphorylated peptides including 22 serine motifs and two threonine motifs (TPE and TP), and 15 motifs from the downregulated phosphorylated peptides including 12 serine motifs and three threonine motifs (TP, RxxT and KxxT) in PYS strain when comparing PYS/PRU. Several kinases were consistent with motifs of overrepresented phosphopeptides, such as PKA, PKG, CKII, IKK, MAPK, EGFR, INSR, Jak, Syk, Src, Ab1. GO analysis, KEGG pathway analysis and STRING analysis revealed differentially expressed phosphoproteins (DEPs) exhibiting significant functional properties. Kinase associated network analysis showed that AGC kinase had the greatest connected peptides. Our findings reveal significant difference in phosphopeptides of sporulated oocysts between virulent and avirulent T. gondii strains, which provides solid foundation for further exploring the mechanisms contributing to the virulence of T. gondii.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:23.040.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ze-Xiang Wang
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-09 01:56:13	ID requested
1	2022-02-22 04:08:19	announced
⏵ 2	2024-10-22 05:33:24	announced	2024-10-22: Updated project metadata.

Wang ZX, Che L, Hu RS, Sun XL, Reveals Stage-Specific Patterns. Molecules, 27(3):(2022) [pubmed]

10.3390/molecules27031022;

curator keyword: Biological, Biomedical

submitter keyword: Phosphoproteomics,Toxoplasma gondii, Sporulated oocyst, Genotype, iBT

Xing-Quan Zhu
contact affiliation	State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
contact email	xingquanzhu1@hotmail.com
lab head
Ze-Xiang Wang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (LVRI, CAAS)
contact email	neau601@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017032
     1. Label: PRIDE project
     2. Name: Comparative phosphoproteomic analysis of sporulated oocysts between virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns