PXD017026

PXD017026 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based characterisation of human megakaryocyte CRLF3 interactome
Description	Cytokine-receptor like factor 3 (CRLF3) is a protein with an as yet unknown function. CRLF3 has been shown to interact with the members of the Hippo pathway in HEK cells but the function of this interaction was not elucidated. Crlf3 null mice are viable and health but present with an isolated reduced platelet count from birth, confirming CRLF3 has a role in platelet production. We performed affinity purification followed by liquid chromatography coupled to tandem mass spectrometry to identify CRLF3’s binding partners in human megakaryocytes.
HostingRepository	PRIDE
AnnounceDate	2022-02-04
AnnouncementXML	Submission_2022-02-04_03:46:31.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cavan Bennett
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-09 00:58:22	ID requested
⏵ 1	2022-02-04 03:46:32	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: CRLF3, LC-MS/MS, megakaryocyte, human

Cedric Ghevaert
contact affiliation	Senior Lecturer in Transfusion Medicine / Consultant Haematologist, University of Cambridge / NHS Blood and Transplant, Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, CB2 0AW, United Kingdom
contact email	cg348@cam.ac.uk
lab head
Cavan Bennett
contact affiliation	Department of Haematology, University of Cambridge
contact email	cavanbennett89@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD017026

PRIDE project URI

• PRIDE
  1. PXD017026
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based characterisation of human megakaryocyte CRLF3 interactome