PXD017025 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive proteomic characterization reveals subclass-specific molecular aberrations within triple-negative breast cancer |
Description | Triple-negative breast cancer (TNBC) is regarded as the most aggressive subtype of breast cancer lacking targeted therapies. This is attributed to its high heterogeneity that complicates elucidation of its molecular aberrations. Here, we report identification of specific proteome expression profiles pertaining to two TNBC subclasses, basal A and basal B, through in-depth proteomics analysis of breast cancer cells. We observed that kinases and proteases displayed unique expression patterns within the subclasses, similar to whole proteome clusters. Systematic analyses of protein-protein interaction and co-regulation networks of these kinases and proteases unraveled dysregulated pathways and plausible targets for each TNBC subclass. Among these, we identified kinases AXL, PEAK1 and TGFBR2, and proteases FAP, UCHL1 and MMP2/14, as specific targets for basal B subclass, which represents the more aggressive TNBC cell lines. Our study thus highlights intricate mechanisms and distinct targets within TNBC, and emphasizes that these have to be exploited in a subclass-specific manner rather than a one-for-all TNBC therapy. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:59:48.532.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Asfa Alli Shaik |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-01-09 00:41:27 | ID requested | |
1 | 2020-03-03 05:47:10 | announced | |
⏵ 2 | 2024-10-22 04:59:50 | announced | 2024-10-22: Updated project metadata. |
Publication List
Kosok M, Alli-Shaik A, Bay BH, Gunaratne J, Comprehensive Proteomic Characterization Reveals Subclass-Specific Molecular Aberrations within Triple-negative Breast Cancer. iScience, 23(2):100868(2020) [pubmed] |
10.1016/j.isci.2020.100868; |
Keyword List
curator keyword: Biomedical |
submitter keyword: triple-negative, TNBC, proteomics, mass spectrometry, protease,Breast cancer, kinase |
Contact List
Jayantha Gunaratne |
contact affiliation | Translational Biomedical Proteomics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore 138673 |
contact email | jayanthag@imcb.a-star.edu.sg |
lab head | |
Asfa Alli Shaik |
contact affiliation | IMCB |
contact email | asfaas@imcb.a-star.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD017025
- Label: PRIDE project
- Name: Comprehensive proteomic characterization reveals subclass-specific molecular aberrations within triple-negative breast cancer