PXD017020

PXD017020 is an original dataset announced via ProteomeXchange.

Title	Crosslinking mass spectrometry (CLMS) analysis of monoubiquitinated FANCD2-FANCI complex
Description	Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer, which then recruits nucleases to remove the DNA lesion. In this study, monoubiquitinated FANCD2-FANCI complex was characterized using crosslinking mass spectrometry in order to provide in sights into the 3D structure of the complex.
HostingRepository	PRIDE
AnnounceDate	2020-03-09
AnnouncementXML	Submission_2020-03-09_04:11:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Chen
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-08 23:50:59	ID requested
⏵ 1	2020-03-09 04:11:31	announced

Alc, ó, n P, Shakeel S, Chen ZA, Rappsilber J, Patel KJ, Passmore LA, FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair. Nat Struct Mol Biol, 27(3):240-248(2020) [pubmed]

curator keyword: Biological

submitter keyword: crosslinking mass spectrometry, FANCD2-FANCI comple, monoubiquitination, fanconi anemia pathway

Juri Rappsilber
contact affiliation	University of Edinburgh; Technische Universit��t Berlin
contact email	Juri.Rappsilber@tu-berlin.de
lab head
Zhuo Chen
contact affiliation	Technischen Universität Berlin
contact email	zhuo.chen@tu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017020
     1. Label: PRIDE project
     2. Name: Crosslinking mass spectrometry (CLMS) analysis of monoubiquitinated FANCD2-FANCI complex