PXD017019

PXD017019 is an original dataset announced via ProteomeXchange.

Title	The role of Bud23 in mouse heart tissue
Description	Efficient mitochondrial function is required in tissues with high energy demand such as the heart, and mitochondrial dysfunction is associated with cardiovascular disease. Expression of mitochondrial proteins is tightly regulated in response to internal and external stimuli. Here we identify a novel mechanism regulating mitochondrial content and function, through BUD23-dependent ribosome generation. BUD23 was required for ribosome maturation, normal 18S/28S stoichiometry and modulated the translation of mitochondrial transcripts in human A549 cells. Deletion of Bud23 in murine cardiomyocytes reduced mitochondrial content and function, leading to severe cardiomyopathy and death. We discovered that BUD23 selectively promotes ribosomal interaction with low GC-content 5’UTRs. Taken together we identify a critical role for BUD23 in bioenergetics gene expression, by promoting efficient translation of mRNA transcripts with low 5’UTR GC content. BUD23 emerges as essential to mouse development, and to postnatal cardiac function.
HostingRepository	PRIDE
AnnounceDate	2020-01-16
AnnouncementXML	Submission_2020-02-17_03:19:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Voronkov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-08 23:51:00	ID requested
1	2020-01-16 00:59:03	announced
⏵ 2	2020-02-17 03:19:40	announced	2020-02-17: Updated publication reference for PubMed record(s): 31939735.

Baxter M, Voronkov M, Poolman T, Galli G, Pinali C, Goosey L, Knight A, Krakowiak K, Maidstone R, Iqbal M, Zi M, Prehar S, Cartwright EJ, Gibbs J, Matthews LC, Adamson AD, Humphreys NE, Rebelo-Guiomar P, Minczuk M, Bechtold DA, Loudon A, Ray D, Cardiac mitochondrial function depends on BUD23 mediated ribosome programming. Elife, 9():(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, Heart, Bud23

David William Ray
contact affiliation	Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, UK
contact email	david.ray@ocdem.ox.ac.uk
lab head
Maria Voronkov
contact affiliation	University of Oxford
contact email	maria.voronkov@stx.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD017019

PRIDE project URI

• PRIDE
  1. PXD017019
     1. Label: PRIDE project
     2. Name: The role of Bud23 in mouse heart tissue