PXD017018

PXD017018 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic profiling of the rat substantia nigra places glial fibrillary acidic protein at the hub of proteins dysregulated during aging: Implications for idiopathic Parkinson’s disease
Description	There is a strong correlation between aging and onset of idiopathic Parkinson’s disease, but little is known about whether cellular changes occur during normal aging that may explain this association. Here, proteomic and bioinformatic analysis was conducted on the substantia nigra of rats at four stages of life to identify and quantify protein changes throughout aging. This analysis revealed that proteins associated with cell adhesion, protein aggregation and oxidative-reduction are dysregulated as early as middle age in rats. Glial fibrillary acidic protein (GFAP) was identified as a network hub connecting the greatest number of proteins altered during aging. Furthermore, the isoform of GFAP expressed in the substantia nigra varied throughout life. However, the expression levels of the rate-limiting enzyme for dopamine production, tyrosine hydroxylase (TH), was maintained even in the oldest animals, despite a reduction in the number of dopamine neurons in the SNc as aging progressed. This age-related increase in TH expression per neuron would likely to increase the vulnerability of neurons, since increased dopamine production would be an additional source of oxidative stress. This, in turn, would place a high demand on support systems from local astrocytes, which themselves show protein changes that could affect their functionality. Taken together, this study highlights key processes that are altered with age in the rat substantia nigra, each of which converge upon GFAP. These findings offer insight into the relationship between aging and increased challenges to neuronal viability, and indicate an important role for glial cells in the aging process.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:15.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sally Shirran
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-08 23:51:00	ID requested
1	2020-03-20 03:33:10	announced
2	2020-04-15 02:48:02	announced	2020-04-15: Updated publication reference for PubMed record(s): 32270889.
⏵ 3	2024-10-22 05:01:20	announced	2024-10-22: Updated project metadata.

G, ó, mez-G, á, lvez Y, Fuller HR, Synowsky S, Shirran SL, Gates MA, Quantitative proteomic profiling of the rat substantia nigra places glial fibrillary acidic protein at the hub of proteins dysregulated during aging: Implications for idiopathic Parkinson's disease. J Neurosci Res, 98(7):1417-1432(2020) [pubmed]

10.1002/jnr.24622;

curator keyword: Biomedical

submitter keyword: proteomics, dopaminergic neuron,glial fibrillary acidic protein, proteome, aging, substantia nigra

Monte Alan Gates
contact affiliation	School of Pharmacy and Bioengineering, Keele University, Staffordshire, UK; and School of Medicine, Keele University, Staffordshire, UK
contact email	m.a.gates@keele.ac.uk
lab head
Sally Shirran
contact affiliation	University of St Andrews
contact email	ss101@st-andrews.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017018
     1. Label: PRIDE project
     2. Name: Quantitative proteomic profiling of the rat substantia nigra places glial fibrillary acidic protein at the hub of proteins dysregulated during aging: Implications for idiopathic Parkinson’s disease