PXD016997

PXD016997 is an original dataset announced via ProteomeXchange.

Title	Identification of proteome changes in apoptotic NSCLC cells
Description	Here, we used an unbiased, functional target-discovery platform to identify immunogenic proteins from primary non-small cell lung cancer (NSCLC) cells that had been induced to apoptosis by cisplatin (CDDP) treatment in vitro, as compared with their live counterparts. Among the multitude of proteins identified, some of them were represented as fragmented proteins in apoptotic tumor cells, and acted as non-mutated neoantigens. Only the fragmented proteins elicited effective multi-specific T cell responses, upon a chemotherapy protocol including CDDP. Importantly, these responses further increased significantly upon anti-PD-1 therapy, and correlated with patients’ survival and decreased PD-1 expression.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_17:12:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessio Grimaldi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-07 07:36:19	ID requested
⏵ 1	2020-05-26 17:12:52	announced

Grimaldi A, Cammarata I, Martire C, Focaccetti C, Piconese S, Buccilli M, Mancone C, Buzzacchino F, Berrios JRG, D'Alessandris N, Tomao S, Giangaspero F, Paroli M, Caccavale R, Spinelli GP, Girelli G, Peruzzi G, Nistic, ò P, Spada S, Panetta M, Letizia Cecere F, Visca P, Facciolo F, Longo F, Barnaba V, Combination of chemotherapy and PD-1 blockade induces T cell responses to tumor non-mutated neoantigens. Commun Biol, 3(1):85(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: NSCLC, SILAC, LC-MSMS

Vincenzo Barnaba
contact affiliation	Dipartimento di Medicina Interna e Specialità Mediche
contact email	vincenzo.barnaba@uniroma1.it
lab head
Alessio Grimaldi
contact affiliation	Dip. Medicina interna e specialità mediche
contact email	alessio.grimaldi@uniroma1.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016997

PRIDE project URI

• PRIDE
  1. PXD016997
     1. Label: PRIDE project
     2. Name: Identification of proteome changes in apoptotic NSCLC cells