PXD016990

PXD016990 is an original dataset announced via ProteomeXchange.

Title	Interspecies assertiveness of Lactobacillus curvatus and Lactobacillus sakei in sausage fermentations
Description	Lactobacillus (L.) curvatus and L. sakei contain strains, which are assertive in sausage fermentation. Previous work has demonstrated differences in assertiveness at strain level within one species, and revealed either exclusion of competitors by complementary partner strains or their inhibition by single strains. This work addresses interspecies differences in the assertiveness of L. curvatus and L. sakei. Strain sets of L. curvatus and L. sakei were employed as starters in a fermented sausage model and their abundancy upon fermentation was determined by strain-specific MALDI-TOF MS identification. Generally, single or groups of L. sakei strains outcompeted L. curvatus strains. In multiple growth tests employing mMRS and mMSM it could be shown that assertive L. sakei strains can be predicted along their µ max in mMSM. Still, L. curvatus TMW 1.624 could suppress all L. curvatus and most L. sakei strains in competitive settings. This could be referred to its expression of several bacteriocins, which are active against all of the L. curvatus strains. Strain specific differences could be demonstrated in the susceptibility of L. sakei to bacteriocins, and in oxidative stress tolerance, which is higher in co-existing L. sakei strains than in the bacteriocin producer. This suggests that tolerance to bacteriocins and oxidative stress represent additional determinants for assertiveness, above previously reported bacteriocin production versus metabolic complementarism of partner strains.
HostingRepository	PRIDE
AnnounceDate	2021-01-07
AnnouncementXML	Submission_2021-01-07_00:12:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Lactobacillus curvatus; NCBI TaxID: 28038;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-07 06:08:50	ID requested
⏵ 1	2021-01-07 00:12:30	announced

Jan, ß, en D, Dworschak L, Ludwig C, Ehrmann MA, Vogel RF, Interspecies assertiveness of Lactobacillus curvatus and Lactobacillus sakei in sausage fermentations. Int J Food Microbiol, 331():108689(2020) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Lactobacillus curvatus, Lactobacillus sakei, fermented sausage, assertiveness, stress tolerance, bacteriocin

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technische Universität München (TUM) Gregor-Mendel-Straße 4 85354 Freising
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016990
     1. Label: PRIDE project
     2. Name: Interspecies assertiveness of Lactobacillus curvatus and Lactobacillus sakei in sausage fermentations