PXD016986

PXD016986 is an original dataset announced via ProteomeXchange.

Title	Metabolic differences between morphologically distinct symbiont populations in the tubeworm Riftia pachyptila
Description	The endosymbiont population of the hydrothermal vent tube worm Riftia pachyptila consists of a single 16S phylotype of sulfur-oxidizing gammaproteobacteria. The intracellular symbiont exhibits remarkable morphological heterogeneity, from small rod-shaped or coccoid cells to large cocci, which were suggested to be part of a common cell cycle. To assess whether these morphological differences are accompanied by distinct metabolic profiles, we physically enriched individual symbiont cells sizes by density gradient centrifugation and subjected these enrichments to metaproteomic analysis and statistical evaluation using clustering and random forests. Unlike previous molecular studies, which examined the metabolism of the symbiont population as whole, we were thus able to unravel comprehensive protein abundance patterns of individual symbiont subpopulations. Supported by microscopic analyses, our metaproteomic results show that Riftia symbiont cells of different sizes are stages of a physiological differentiation process: Small symbionts actively divide and may establish cellular symbiont-host interaction, as indicated by highest abundance of the cell division key protein FtsZ and highly abundant chaperones and porins in this initial phase. We furthermore present evidence that large symbionts, on the other hand, do not divide, but still replicate DNA, leading to DNA endoreduplication. Highest abundance of enzymes for CO2 fixation, carbon storage and biosynthesis indicates that in its late differentiation stage, the symbiont’s metabolism is efficiently geared on the production of organic material. We propose that this symbiont aging process enhances the productivity of the symbiosis as a whole.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:00.800.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tjorven Hinzke
SpeciesList	scientific name: Candidatus Endoriftia persephone; NCBI TaxID: 393765; scientific name: Riftia pachyptila; NCBI TaxID: 6426;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-07 04:51:57	ID requested
1	2021-03-04 10:54:22	announced
⏵ 2	2024-10-22 05:17:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: mutualism,Metaproteomics, symbiosis, deep sea, hydrothermal vents, holobiont, gradient centrifugation, mutualistic association

Stephanie Markert
contact affiliation	Institute of Pharmacy, University of Greifswald, Germany Institute of Marine Biotechnology, Greifswald, Germany
contact email	stephanie.markert@uni-greifswald.de
lab head
Tjorven Hinzke
contact affiliation	University of Greifswald, Greifswald Intitute of Marine Biotechnology
contact email	tjorven.hinzke@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD016986

PRIDE project URI

• PRIDE
  1. PXD016986
     1. Label: PRIDE project
     2. Name: Metabolic differences between morphologically distinct symbiont populations in the tubeworm Riftia pachyptila