PXD016983

PXD016983 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomic and Metabolomic analysis to study the effects of spaceflight on Candida albicans
Description	Background: Candida albicans is an opportunistic pathogenic yeast, which could become pathogenic in various stressful environmental factors including the spaceflight environment. In this study,we aim to explore the phenotypic changes and possible mechanisms of Candida albicans after exposure to spaceflight conditions. Results: The effect of Candida albicans after carried on the "SJ-10" satellite for 12 days was evaluated by proliferation, morphogenic, environmental resistance and virulence experiment. The result showed that the proliferation rate, biofilm formation, antioxidant capacity, cytotoxicity and filamentous morphology of Candida albicans were increased in the spaceflight group compared to the control group. Proteomics and metabolomics technology were used to analyze the profiles of proteins and metabolites in Candida albicans under spaceflight condition. Proteomic analysis identified 564 up-regulated proteins involved in ribosome, DNA replication, base excision repair and sulfur metabolism in spaceflight group. And 345 down-regulated proteins related to metabolic processes were observed. The metabolomic analysis found 5 different expressed metabolites. The combined analysis of proteomic and metabolomic revealed the accumulation of cysteine and methionine in Candida albicans after spaceflight. Conclusions: Mechanisms that could explain the results in phenotype experiment of Candida albicans were found through proteomic and metabolomic analysis. And our data provide an important basis for the assessment of the risk that Candida albicans could cause under spaceflight environment.
HostingRepository	iProX
AnnounceDate	2020-08-27
AnnouncementXML	Submission_2020-10-08_22:39:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongzhi Li
SpeciesList	scientific name: Candida albicans; NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-07 04:12:08	ID requested
1	2020-08-26 19:55:52	announced
⏵ 2	2020-10-08 22:39:45	announced	2020-10-09: Update publication information

Wang J, Liu Y, Zhao G, Gao J, Liu J, Wu X, Xu C, Li Y, Integrated proteomic and metabolomic analysis to study the effects of spaceflight on Candida albicans. BMC Genomics, 21(1):57(2020) [pubmed]

submitter keyword: Spaceflight, Metabolomics, Proteomics, Candida albicans

Yongzhi Li
contact affiliation	Astronaut Center of China
contact email	liyongzhi1111@163.com
lab head
Yongzhi Li
contact affiliation	Astronaut Center of China
contact email	liyongzhi1111@163.com
dataset submitter

iProX dataset URI