PXD016970

PXD016970 is an original dataset announced via ProteomeXchange.

Title	Compositional and Immunogenic Evaluation of Fractionated Wheat Beers using Mass Spectrometry
Description	The safety and regulatory status of fermented products derived from gluten-containing grains for patients with celiac disease has remained controversial. Bottom-up mass spectrometry (MS) has complemented immunoassays for the compositional and immunogenic analyses of wheat beers. However, uncharacterized proteolysis during brewing followed by the secondary digestion for MS analysis has made the analysis and data interpretation complicated. In this study, the composition and immunogenic potential of seven commercially available wheat beers were evaluated using bottom-up MS with the aid of fractionation and a multi-step peptide search strategy to capture possible cleavage combinations. Gluten-derived peptides accounted for approximately 50% and 20% of the total number of wheat-derived and barley-derived peptides in investigated beers, respectively. Although relatively large polypeptides cannot be thoroughly characterized using traditional bottom-up proteomics, up to 50% of the identified peptides still contained celiac-immunogenic motifs, and consumption of wheat beers would be risky and not recommended for celiac patients.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:09:19.846.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanying Cao
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-06 05:44:08	ID requested
1	2020-07-16 18:04:08	announced
⏵ 2	2024-10-22 05:09:27	announced	2024-10-22: Updated project metadata.

Cao W, Baumert JL, Downs ML, Compositional and immunogenic evaluation of fractionated wheat beers using mass spectrometry. Food Chem, 333():127379(2020) [pubmed]

10.1016/j.foodchem.2020.127379;

curator keyword: Biological

submitter keyword: Gluten, Celiac disease,Wheat beers, Mass spectrometry, Proteomics, Fermentation

Melanie L. Downs
contact affiliation	FARRP, UNL 1901 North 21st Street, University of Nebraska-Lincoln Lincoln, NE 68588-6205
contact email	mdowns2@unl.edu
lab head
Wanying Cao
contact affiliation	UNL
contact email	wanying.cao@huskers.unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016970

PRIDE project URI

• PRIDE
  1. PXD016970
     1. Label: PRIDE project
     2. Name: Compositional and Immunogenic Evaluation of Fractionated Wheat Beers using Mass Spectrometry