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PXD016960

PXD016960 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNanoTPOT: Enhanced Sample Preparation for Quantitative Nanoproteomic Analysis
DescriptionIn this study, we developed and optimized a nanoproteomic workflow that we termed Nanogram TMT Processing in One Tube (NanoTPOT). Through the assessment of proteolytic digestion, tandem mass tag (TMT) labeling, online and offline frac-tionation strategies, our optimized workflow effectively eliminated the need for sample desalting and enabled compatible sample processing for MS analysis. We further applied the NanoTPOT workflow to examine cellular response to stress caused by dithiothreitol in Hela cells, where we identified and quantified 6935 proteins in a TMT 10-plex experiment with one microgram of starting material in each channel.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:17:44.835.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYu Lu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-06 05:15:50ID requested
12021-01-11 00:22:52announced
22024-10-22 05:17:45announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.analchem.0c00077;
Wu R, Pai A, Liu L, Xing S, Lu Y, NanoTPOT: Enhanced Sample Preparation for Quantitative Nanoproteomic Analysis. Anal Chem, 92(9):6235-6240(2020) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Hela, 2D LC-MS,TMT
Contact List
Yu Lu
contact affiliationDepartment of Biochemistry and Biomedical Sciences McMaster University
contact emailyu.lu@mcmaster.ca
lab head
Yu Lu
contact affiliationMcMaster University
contact emailyu.lu@mcmaster.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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