PXD016960

PXD016960 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	NanoTPOT: Enhanced Sample Preparation for Quantitative Nanoproteomic Analysis
Description	In this study, we developed and optimized a nanoproteomic workflow that we termed Nanogram TMT Processing in One Tube (NanoTPOT). Through the assessment of proteolytic digestion, tandem mass tag (TMT) labeling, online and offline frac-tionation strategies, our optimized workflow effectively eliminated the need for sample desalting and enabled compatible sample processing for MS analysis. We further applied the NanoTPOT workflow to examine cellular response to stress caused by dithiothreitol in Hela cells, where we identified and quantified 6935 proteins in a TMT 10-plex experiment with one microgram of starting material in each channel.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:44.835.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-06 05:15:50	ID requested
1	2021-01-11 00:22:52	announced
⏵ 2	2024-10-22 05:17:45	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acs.analchem.0c00077;
Wu R, Pai A, Liu L, Xing S, Lu Y, NanoTPOT: Enhanced Sample Preparation for Quantitative Nanoproteomic Analysis. Anal Chem, 92(9):6235-6240(2020) [pubmed]

10.1021/acs.analchem.0c00077;

Wu R, Pai A, Liu L, Xing S, Lu Y, NanoTPOT: Enhanced Sample Preparation for Quantitative Nanoproteomic Analysis. Anal Chem, 92(9):6235-6240(2020) [pubmed]

Keyword List

curator keyword: Technical
submitter keyword: Hela, 2D LC-MS,TMT

curator keyword: Technical

submitter keyword: Hela, 2D LC-MS,TMT

Contact List

Yu Lu
contact affiliation	Department of Biochemistry and Biomedical Sciences McMaster University
contact email	yu.lu@mcmaster.ca
lab head
Yu Lu
contact affiliation	McMaster University
contact email	yu.lu@mcmaster.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD016960
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD016960

PRIDE project URI

Repository Record List

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