PXD016958

PXD016958 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of microdissected renal tubules
Description	Kidney is a vital organ which help maintain body homeostasis. Three related processes including glomerular filtration, tubular reabsorption and secretion are precisely regulated in the kidney in response to changes in the amount of both water and various solutes. To understand how the kidney can achieve this challenging goal, it is important to know the protein composition in the kidney. However, unlike other solid organs, kidney has a very intricate architecture, including at least 14 distinct tubule segments. This reason limits the interpretibility of experiments perfomred using whole kidney homegenate samples. To mitigate this problem, we combined kidney tubule microdissection technique with protein mass spectrometry to study the proteome of 14 individual rat kidney tubule segment.
HostingRepository	PRIDE
AnnounceDate	2020-04-20
AnnouncementXML	Submission_2020-07-14_23:32:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kavee Limbutara
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-06 05:13:28	ID requested
1	2020-04-20 00:02:42	announced
⏵ 2	2020-07-14 23:32:23	announced	2020-07-15: Updated publication reference for PubMed record(s): 32358040.

Limbutara K, Chou CL, Knepper MA, Quantitative Proteomics of All 14 Renal Tubule Segments in Rat. J Am Soc Nephrol, 31(6):1255-1266(2020) [pubmed]

curator keyword: Biological

submitter keyword: Kidney, Microdissection, Physiology, LC-MS/MS, Renal tubule

Mark A. Knepper
contact affiliation	Epithelial Systems Biology Laboratory, Systems Biology Center, NHLBI, NIH
contact email	knepperm@nhlbi.nih.gov
lab head
Kavee Limbutara
contact affiliation	Epithelial Systems Biology Laboratory, Systems Biology Center, NHLBI, NIH
contact email	kavee.limbutara@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD016958

PRIDE project URI

• PRIDE
  1. PXD016958
     1. Label: PRIDE project
     2. Name: Proteomic analysis of microdissected renal tubules