PXD016944

PXD016944 is an original dataset announced via ProteomeXchange.

Title	Quick steps to get glioma extracellular vesicles surface membrane proteins mapping based on tryspin/Lysin C digestion followed by Amicon and shot gun proteomic: identification of ghost protein
Description	Extracellular vesicles (EVs) are known to mediate intercellular communication and regulate a diverse range of crucial biological processes. Novel therapeutic strategies have emerged based on these EVs as biological nanoparticles. EVs is as a generic term for all secreted vesicles released including at least ectosomes, microvesicles, apoptotic bodies and exosomes. In order to determine if it is possible to get access to the protein present at the surface of the EVs, we developed first a quickstep strategy. We performed a Trypsin/Lys C digestion treatment for EVs pellet, followed by an Amicon filtration. This procedure sep-arates the EVs from peptides of protein aggregates and external part of EVs membrane proteins. After separation step, all the fractions have been subjected of proteomic analyses. Comparison between T-0h and T-6h, Trypsin/Lysin C digestion revealed an evolution of the surface EVs proteins. Some surface proteins have been demasked after 6h enzymatic digestion like CD81, CD82 ,Ust, Vcan, Lamp 1,rab43, Cspg4, Annexin A2, Synthenin 1,VSP37b. These results established an evolu-tion in time course of the nature of the surface proteins in glioma tumor.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:21.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soulaimane Aboulouard
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-06 02:06:09	ID requested
1	2021-02-12 06:18:46	announced
⏵ 2	2023-11-14 08:50:22	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: glioma, vesicle, ghost-protein,LC-MSMS

Michel Salzet
contact affiliation	Laboratoire PRISM INSERM U-1192 Universite de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Soulaimane Aboulouard
contact affiliation	PRISM
contact email	soulaimane.aboulouard@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD016944

PRIDE project URI

• PRIDE
  1. PXD016944
     1. Label: PRIDE project
     2. Name: Quick steps to get glioma extracellular vesicles surface membrane proteins mapping based on tryspin/Lysin C digestion followed by Amicon and shot gun proteomic: identification of ghost protein