PXD016928

PXD016928 is an original dataset announced via ProteomeXchange.

Title	Proteome analyses of sperm from wild type, Akap3 and Akap4 gene null mutant mice
Description	PKA signaling plays important functions during mouse spermatogenesis and for the regulation of sperm motility. The efficiency of PKA signaling is regulated partly by its binding protein A-kinase anchoring protein (AKAP) family. Mature mouse sperm contain several AKAPs, of which AKAP3 and AKAP4 are localized in the principal piece of sperm tail and they are sperm-specific. Using gene knock-out mouse models, it was found that both AKAP3 and AKAP4 are essential for the formation of a complete fibrous sheath, the electron-dense sub-cellular structure within sperm flagellum that is important for the motility of sperm and male fertility. Lack of AKAP3 or AKAP4 caused aberrant sperm morphology and inmotility, leading to eventual male sterility. In the present study, we investigated the changes of cellular proteome in the absence of AKAP3 or AKAP4 in mature sperm, using quantitative mass spectrometry. It was found that global changes of sperm proteome occur in the absence of AKAP3 or AKAP4. In Akap3 null sperm, proteins related to flagella structure, PKA signaling and motility regulations are down-regulated, whereas proteins involved in RNA metabolism, protein translation and actin filaments formation are accumulated. Akap4 null sperm also displayed changes of sperm proteome at lesser extends. These results suggest that both AKAP3 and AKAP4 are essential for the formation of sperm sub-cellular structures, proper regulation of PKA activity and motility of sperm, providing new clues to elucidate the molecular pathways that underlie the coordinated synthesis and organization of sperm proteome and sperm morphology
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:06.048.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huayu Qi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-02 05:24:28	ID requested
1	2020-01-14 00:00:23	announced
2	2020-01-29 06:25:24	announced	2020-01-29: Updated publication reference for PubMed record(s): 31969357.
3	2020-02-17 03:18:16	announced	2020-01-29: Updated publication reference for PubMed record(s): 31969357. 2020-02-17: Updated publication reference for PubMed record(s): 31969357.
⏵ 4	2024-10-22 04:10:10	announced	2024-10-22: Updated project metadata.

10.1242/dev.181057;

Xu K, Yang L, Zhang L, Qi H, Lack of AKAP3 disrupts integrity of the subcellular structure and proteome of mouse sperm and causes male sterility. Development, 147(2):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Mouse sperm

spermiogenesis

AKAP3

AKAP4

Fibrous sheath

Huayu Qi
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	qi_huayu@gibh.ac.cn
lab head
Huayu Qi
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	qi_huayu@gibh.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016928

PRIDE project URI

• PRIDE
  1. PXD016928
     1. Label: PRIDE project
     2. Name: Proteome analyses of sperm from wild type, Akap3 and Akap4 gene null mutant mice