PXD016927

PXD016927 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of global protein acetylation in PRRSV infected pulmonary alveolar macrophages
Description	Porcine reproductive and respiratory syndrome (PRRS), which caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is a serious viral disease affecting global swine industry. At present, PRRSV vaccines fail to prevent this disease. Consequently, new antiviral strategies to compensate for the inefficacy of available vaccines are urgently required. Lysine acetylation is an important post-translational modification (PTM) regulating an array of pathological and physiological conditions. In this study, we profiled the global acetylome using acetylation specific antibody based enrichment and Tandem mass tag (TMT) label LC-MS in PRRSV-infected pulmonary alveolar macrophages (PAMs). As a result, 3731 lysine acetylation sites on 1421 cellular proteins were identified and quantified 6 hours post infection (hpi). Bioinformatics analysis of the differentially acetylated proteins revealed their involvement in various biological processes, including the host immune response and energy metabolism.
HostingRepository	PRIDE
AnnounceDate	2020-12-11
AnnouncementXML	Submission_2020-12-10_23:18:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianyu Fang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-01-02 05:24:17	ID requested
⏵ 1	2020-12-10 23:18:30	announced

Fang J, Qiao S, Wang K, Li R, Wang L, Li H, Zhang G, Quantitative Proteomic Analysis of Global Protein Acetylation in PRRSV-Infected Pulmonary Alveolar Macrophages. Proteomics, 21(2):e2000019(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: Porcine reproductive and respiratory syndrome

Tandem mass tag label LC-MS

post-translational modification

acetylation.

Gaiping Zhang
contact affiliation	Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
contact email	zhanggaip@126.com
lab head
Jianyu Fang
contact affiliation	Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences
contact email	fangjianyu2006@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD016927

PRIDE project URI

• PRIDE
  1. PXD016927
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of global protein acetylation in PRRSV infected pulmonary alveolar macrophages