PXD016880 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication |
Description | Hepatitis Delta virus (HDV) is a satellite of Hepatitis B virus with a single stranded circular RNA genome. HDV RNA genome synthesis is carried out in infected cells by cellular RNA polymerases with the assistance of the small hepatitis delta antigen (S-HDAg). Here we show that S-HDAg binds the Bromodomain (BRD) Adjacent To Zinc Finger Domain 2B (BAZ2B) protein, a regulatory subunit of BRF (BAZ2B-Associated Remodeling Factor) ISWI chromatin remodeling complexes. ShRNAs-mediated silencing of BAZ2B or its inactivation with the BAZ2B-BRD inhibitor GSK-2801 impairs HDV replication in HDV-infected human hepatocytes. S-HDAg contains a short linear interacting motif (SLiM) KacXXR, similar to the one recognized by BAZ2B-BRD in histone H3. We found that the integrity of the S-HDAg SLiM sequence is required for S-HDAg interaction with BAZ2B-BRD and for HDV RNA replication. Our results suggest that S-HDAg uses a histone mimicry strategy to co-activate the RNA Polymerase II-dependent synthesis of HDV RNA and sustain HDV replication. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:05:58.353.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Dény |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-20 06:46:05 | ID requested | |
1 | 2020-05-26 16:41:15 | announced | |
⏵ 2 | 2024-10-22 05:06:00 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-020-14299-9; |
Abeywickrama-Samarakoon N, Cortay JC, Sureau C, M, ü, ller S, Alfaiate D, Guerrieri F, Chaikuad A, Schr, ö, der M, Merle P, Levrero M, D, é, ny P, Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication. Nat Commun, 11(1):419(2020) [pubmed] |
Keyword List
submitter keyword: Short Linear Interaction Motif (SLiM),Hepatitis Delta Virus (HDV), SNF2H / SMARCA-5, SNF2L / SMARCA¬1, BAZ2B Associated Remodeling Factor (BRF), RNA Polymerase II (RNA Pol II), Histone Mimicry, Hepatitis B Virus (HBV), Bromodomain Associated To Zinc Finger Protein 2B (BAZ2B) |
Contact List
Pr. Massimo Levrero |
contact affiliation | CRCL UMR Inserm U1052, CNRS 5286, UCBL, CLB |
contact email | massimo.levrero@inserm.fr |
lab head | |
Paul Dény |
contact affiliation | Université Paris 13, Unité de Formation et Recherches Santé Médecine et Biologie Humaine, BOBIGNY Centre de Recherches en Cancérologie de Lyon, INSERM U1052, UMR CNRS 5268LYON |
contact email | paul.deny@inserm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016880
- Label: PRIDE project
- Name: Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication