PXD016880

PXD016880 is an original dataset announced via ProteomeXchange.

Title	Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication
Description	Hepatitis Delta virus (HDV) is a satellite of Hepatitis B virus with a single stranded circular RNA genome. HDV RNA genome synthesis is carried out in infected cells by cellular RNA polymerases with the assistance of the small hepatitis delta antigen (S-HDAg). Here we show that S-HDAg binds the Bromodomain (BRD) Adjacent To Zinc Finger Domain 2B (BAZ2B) protein, a regulatory subunit of BRF (BAZ2B-Associated Remodeling Factor) ISWI chromatin remodeling complexes. ShRNAs-mediated silencing of BAZ2B or its inactivation with the BAZ2B-BRD inhibitor GSK-2801 impairs HDV replication in HDV-infected human hepatocytes. S-HDAg contains a short linear interacting motif (SLiM) KacXXR, similar to the one recognized by BAZ2B-BRD in histone H3. We found that the integrity of the S-HDAg SLiM sequence is required for S-HDAg interaction with BAZ2B-BRD and for HDV RNA replication. Our results suggest that S-HDAg uses a histone mimicry strategy to co-activate the RNA Polymerase II-dependent synthesis of HDV RNA and sustain HDV replication.
HostingRepository	PRIDE
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2020-05-26_16:41:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Dény
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-20 06:46:05	ID requested
⏵ 1	2020-05-26 16:41:15	announced

Abeywickrama-Samarakoon N, Cortay JC, Sureau C, M, ü, ller S, Alfaiate D, Guerrieri F, Chaikuad A, Schr, ö, der M, Merle P, Levrero M, D, é, ny P, Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication. Nat Commun, 11(1):419(2020) [pubmed]

submitter keyword: Hepatitis Delta Virus (HDV), Hepatitis B Virus (HBV), RNA Polymerase II (RNA Pol II), Histone Mimicry, Short Linear Interaction Motif (SLiM), Bromodomain Associated To Zinc Finger Protein 2B (BAZ2B), BAZ2B Associated Remodeling Factor (BRF), SNF2L / SMARCA¬1, SNF2H / SMARCA-5

Pr. Massimo Levrero
contact affiliation	CRCL UMR Inserm U1052, CNRS 5286, UCBL, CLB
contact email	massimo.levrero@inserm.fr
lab head
Paul Dény
contact affiliation	Université Paris 13, Unité de Formation et Recherches Santé Médecine et Biologie Humaine, BOBIGNY Centre de Recherches en Cancérologie de Lyon, INSERM U1052, UMR CNRS 5268LYON
contact email	paul.deny@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD016880

PRIDE project URI

• PRIDE
  1. PXD016880
     1. Label: PRIDE project
     2. Name: Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication