PXD016876

PXD016876 is an original dataset announced via ProteomeXchange.

Title	Drosophila melanogaster lamellocytes proteome
Description	In Drosophila melanogaster larval hemolymph, under normal conditions, plasmatocytes and crystal cells represent respectively ~95% and ~5% of hemocytes, while lamellocytes, the third larval cell type, are absent since they are only induced after parasitoid wasp oviposition, their role being the encapsulation-melanization response to eliminate the wasp egg. However, even after induction lamellocytes number remains low, making difficult biochemical studies. Here using the D. melanogaster hopTum-l mutant that constitutively produces a high number of hemocytes, we set up a method to purify lamellocytes and analyzed their major proteins by 2D gel electrophoresis and their biotinylated plasma membrane surface proteins by 1D SDS-PAGE after affinity purification. Mass spectrometry allowed to identify 430 proteins from the 2D spots and 344 from affinity purified proteins, totalizing 639 unique proteins. Known lamellocyte markers such as PPO3 and the integrin myospheroid are among the major proteins and affinity purification led to the detection of other integrins and a large array of integrins associated proteins involved in cell-cell junction formation and function. Overall newly identified proteins indicated that these cells are highly adapted to the encapsulation process but may have also several different physiological functions. This study provides the basis for new lamellocyte studies in vivo and in vitro, and develop markers to search whether different populations coexist, establish their origins and decipher their respective roles in drosophila physiology and immunity.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:35:00.276.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD016876
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maya BELGHAZI
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-20 03:50:54	ID requested
⏵ 1	2024-10-07 10:35:05	announced

10.6019/PXD016876;

10.1016/j.ibmb.2021.103584;

Wan B, Belghazi M, Lemauf S, Poiri, é M, Gatti JL, identifies new membrane proteins and networks involved in their functions. Insect Biochem Mol Biol, 134():103584(2021) [pubmed]

submitter keyword: membrane proteins, hemocytes purification,Drosophila melanogaster, lamellocytes, Biotin-avidin protein purification

Jean Luc GATTI
contact affiliation	Evolution and Specificity of Multitrophic Interactions (ESIM) Sophia Agrobiotech Institute (ISA) INRA / CNRS / Université de Nice 400 route des Chappes BP 167 06903 Sophia Antipolis Cedex - France
contact email	Jean-Luc.gatti@inra.fr
lab head
Maya BELGHAZI
contact affiliation	CNRS
contact email	maya.belghazi@univ-amu.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD016876

PRIDE project URI

• PRIDE
  1. PXD016876
     1. Label: PRIDE project
     2. Name: Drosophila melanogaster lamellocytes proteome