PXD016848

PXD016848 is an original dataset announced via ProteomeXchange.

Title	quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7
Description	Clostridium sp. strain CT7 is a new emerging microbial cell factory with high butanol ratio owing to the non-traditional butanol fermentation mode with uncoupled acetone and 1,3-propanediol formation. Significant change of products profile was shown in glycerol- and glucose-fed strain CT7, especially much higher butanol and lower volatile fatty acids production from glycerol-fed one. However, the mechanism of this interesting phenomenon was still unclear. To better elaborate the bacterial response towards glycerol and glucose, the quantitative proteomic analysis through iTRAQ strategy was performed to reveal the regulated proteomic expression levels under different substrates. Proteomics data showed highly increased proteomic expression levels of proteins related with glycerol utilization and solvent generation under glycerol media. In addition, the up-regulation of hydrogenases, ferredoxins and electron-transferring proteins may attribute to the internal redox balance, while the earlier triggered sporulation response in glycerol-fed media may be associated with the higher butanol fermentation. This study will provide the platform for metabolic engineering of this emerging industrial microorganism for more efficient butanol production from glycerol.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_08:10:23.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaonan fu
SpeciesList	scientific name: uncultured Clostridium sp.; NCBI TaxID: 59620;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-19 06:25:22	ID requested
⏵ 1	2021-09-08 08:10:24	announced

Jiang Y, Wu R, Lu J, Dong W, Zhou J, Zhang W, Xin F, Jiang M, Quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7. Microb Cell Fact, 20(1):12(2021) [pubmed]

curator keyword: Technical

submitter keyword: iTRAQ

proteomic analysis

glycerol

butanol

Clostridium

Fengxue Xin
contact affiliation	College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University
contact email	1515628407@qq.com
lab head
xiaonan fu
contact affiliation	zhejiang scientific technology universitiy
contact email	2016460649@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016848
     1. Label: PRIDE project
     2. Name: quantitative proteomic analysis to reveal expression differences for butanol production from glycerol and glucose by Clostridium sp. strain CT7