PXD016837

PXD016837 is an original dataset announced via ProteomeXchange.

Title	The melanoma cell line MDA-MB-435 adhesome in long-term culture and its comparison to the adhesome upon integrin αV knockdown
Description	Using mass spectrometry–based proteomics, we analysed the components of integrin adhesion complexes of MDA-MB-435S cells and two MDA-MB-435S-derived integrin αV-specific shRNA-expressing cell clones. Among integrins, we detected αV and β5 integrin subunits in the MDA-MB-435S adhesome, thus showing that in long term culture these cells use preferentially integrin αVβ5 for adhesion. Our data represents the first published adhesome of αVβ5 integrin heterodimer.
HostingRepository	PRIDE
AnnounceDate	2020-02-17
AnnouncementXML	Submission_2020-05-18_06:07:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mladen Paradzik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-19 05:44:02	ID requested
1	2020-02-17 08:33:05	announced
⏵ 2	2020-05-18 06:07:10	announced	2020-05-18: Updated publication reference for PubMed record(s): 32195252.

Parad, ž, ik M, Humphries JD, Stojanovi, ć N, Nesti, ć D, Majhen D, Dekani, ć A, Samar, ž, ija I, Sedda D, Weber I, Humphries MJ, Ambriovi, ć, -Ristov A, 5 Focal Adhesions to Microtubules and Regulates Sensitivity to Microtubule Poisons and Cell Migration. Front Cell Dev Biol, 8():125(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, cancer, melanoma, adhesome, integrin αVβ5, cortical microtubule stabilisation complex, integrin adhesion complex, MDA-MB-435S

Andreja Ambriović-Ristov
contact affiliation	Laboratory for Cell Biology and Signalling, Division of Molecular Biology, Ruder Boskovic Institute, Zagreb, Croatia
contact email	Andreja.Ambriovic.Ristov@irb.hr
lab head
Mladen Paradzik
contact affiliation	Ruder Boskovic Institute
contact email	mladen.paradzik@irb.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD016837

PRIDE project URI

• PRIDE
  1. PXD016837
     1. Label: PRIDE project
     2. Name: The melanoma cell line MDA-MB-435 adhesome in long-term culture and its comparison to the adhesome upon integrin αV knockdown